Various culture media have been used to maintain the AGS cell line in culture and for specific experimental procedures. A 10% fetal bovine serum (FBS)-supplemented RPMI medium is the most popular medium used by many researchers to maintain AGS cells in culture. Some have also used 10% FBS-supplemented DMEM. ATCC suggests Ham’s F12K Medium supplemented with 10% FBS. A list of base media used for AGS cell line, experiment-specific supplements, culture conditions, purpose, and references are given in the table below.
| Culture medium (Base medium) | Supplements | Culture conditions | Purpose | References |
| Ham’s F-12K | 10% FBS | 37°C, 95% Air + 5% CO2 humidified atmosphere | Cell culture maintenance | ATCC |
| Ham’s F12K | 10% FBS + 2 mM L-glutamine + 1.5 g/L sodium bicarbonate | 37°C, 95% Air + 5% CO2 humidified atmosphere | Cell culture maintenance | HMS LINCS DB |
| Ham’s F12 | 10% FBS + 2mM Glutamine | 37°C, 95% Air + 5% CO2 humidified atmosphere | Cell culture maintenance | Culture Collections, UK Health Security Agency; CellBank Australia; Sigmaaldrich |
| RPMI-1640 | 10% hiFBS + L-glutamine (300 mg/L) + 25mM HEPES + 25mM NaHCO3 | 37°C, 95% Air + 5% CO2 humidified atmosphere | Cell culture maintenance | Korean Cell Line Bank |
| RPMI-1640 | 10% FBS | 37°C, 95% Air + 5% CO2 humidified atmosphere | Cell culture maintenance | Kim et al., 2013 |
| RPMI-1640 | 10% hiFBS, 2 mM glutamine + 100 U/ml penicillin + 100 μg/ml streptomycin | 37°C, 95% Air + 5% CO2 humidified atmosphere | Cell culture maintenance | (Park et al., 2015) |
| RPMI-1640 | 10% FBS + 100 U/l penicillin + 100 mg/l streptomycin | 37°C, 95% Air + 5% CO2 humidified atmosphere | Cell culture maintenance | (Kim et al., 2013) |
| RPMI-1640 | 10% FBS + 10 IU/l penicillin + 10 mg/l streptomycin | 37°C, 95% Air + 5% CO2 humidified atmosphere | Cell culture maintenance | (Karayiannis et al., 2023) |
| DMEM | 10% FBS + 2 mM L -glutamine + 4.5 g/L glucose | 37°C, 95% Air + 5% CO2 humidified atmosphere | Cell culture maintenance | CLS Cell Lines Service |
| DMEM | 10% FBS + 10 μg/ml vancomycin | 37°C, 95% Air + 5% CO2 humidified atmosphere | Cell culture maintenance | Bourzac et al., 2007 |
| RPMI1640 | No FBS | 37°C, 95% Air + 5% CO2 humidified atmosphere | Serum-starvation studies, Multinuclear giant cell formation studies | Kim et al., 2005) |
| DMEM | 10% FBS + 10 μg/ml vancomycin + 10% brucella broth | Helicobacter pylori infection | Bourzac et al., 2007 |
Abbreviations:
- DMEM (Dulbecco’s modified Eagle’s medium)
- RPMI 1640 (Roswell Park Memorial Institute 1640)
- FBS (Fetal Bovine Serum)
- hiFBS (Heat-inactivated FBS)
- ATCC (American Type Culture Collection)
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