AGS Cell Line: Culture Media

Various culture media have been used to maintain the AGS cell line in culture and for specific experimental procedures. A 10% fetal bovine serum (FBS)-supplemented RPMI medium is the most popular medium used by many researchers to maintain AGS cells in culture. Some have also used 10% FBS-supplemented DMEM. ATCC suggests Ham’s F12K Medium supplemented with 10% FBS (atcc). A list of base media used for AGS cell line, experiment-specific supplements, culture conditions, purpose, and references are given in the table below.

Culture medium (Base medium)SupplementsCulture conditionsPurposeReferences
Ham’s F-12K10% FBS37°C95% Air + 5% CO2 humidified atmosphereCell culture maintenanceATCC
Ham’s F12K10% FBS + 2 mM L-glutamine +  1.5 g/L sodium bicarbonate37°C95% Air + 5% CO2 humidified atmosphereCell culture maintenanceHMS LINCS DB
Ham’s F1210% FBS + 2mM Glutamine37°C95% Air + 5% CO2 humidified atmosphereCell culture maintenanceCulture Collections, UK Health Security Agency; CellBank Australia; Sigmaaldrich
RPMI1640 10% hiFBS + L-glutamine (300 mg/L) + 25mM HEPES + 25mM NaHCO337°C95% Air + 5% CO2 humidified atmosphereCell culture maintenanceKorean Cell Line Bank
RPMI164010% FBS37°C95% Air + 5% CO2 humidified atmosphereCell culture maintenanceKim et al., 2013
RPMI164010% hiFBS, 2 mM glutamine + 100 U/ml penicillin + 100 μg/ml streptomycin37°C95% Air + 5% CO2 humidified atmosphereCell culture maintenance(Park et al., 2015)
RPMI-1640 10% FBS + 100 U/l penicillin + 100 mg/l streptomycin.37°C95% Air + 5% CO2 humidified atmosphereCell culture maintenance(Kim et al., 2013)
DMEM10% FBS + 2 mM L -glutamine + 4.5 g/L glucose37°C95% Air + 5% CO2 humidified atmosphereCell culture maintenanceCLS Cell Lines Service
DMEM10% FBS + 10 μg/ml vancomycin37°C95% Air + 5% CO2 humidified atmosphereCell culture maintenanceBourzac et al., 2007
     
RPMI1640No FBS37°C95% Air + 5% CO2 humidified atmosphereSerum-starvation studies, Multinuclear giant cell formation studiesKim et al., 2005)
DMEM10% FBS + 10 μg/ml vancomycin + 10% brucella broth Helicobacter pylori infectionBourzac et al., 2007

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