Tag: escherichia coli

Escherichia coli Pathogenic Strains

Pathogenic strains of Escherichia coli represent distinct variants that have acquired specific virulence factors through horizontal gene transfer and evolutionary…

Escherichia coli in Research: Timeline of Discovery and Innovation

1885: Theodor Escherich isolates and describes Bacterium coli commune (later renamed Escherichia coli) from infant stool samples. This discovery marks…

Laboratory Strains of Escherichia coli

Laboratory strains of Escherichia coli represent specialized variants that have been developed and optimized for research and biotechnology applications. These…

Escherichia coli

Escherichia coli, commonly referred to as E. coli, is a Gram-negative, facultative anaerobic bacterium that belongs to the Enterobacteriaceae family. …

Growing Escherichia coli for Plasmid Isolation

Amplification of plasmid is desirable for many applications including gene cloning, DNA sequencing, transfection, and probe preparation. The fastest and routinely used method to amplify plasmid is to introduce plasmid in an appropriate strain of E. coli e.g., DH5α (the process is called transformation), grow them to a suitable culture volume, and finally, extract plasmid from them (the process called plasmid isolation).