Transfection is a very common and powerful method to deliver nucleic acid (DNA/RNA) into tissue culture cells. Usually, the purpose is to manipulate the gene expression of cells to study the function and regulation of a gene(s). It can also be used to overexpress a protein and purify it. 

Transfection can be used to 

  • Overexpression of gene
  • Downregulation of gene
  • Creating mutations or deletion
  • Homologous recombination
  • Analysis of promoter activity (reporter assay)

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