- Synonyms: 293 GTP-AC-free
- Description:
- Original cell line: 293 (also known as HEK-293)
- 293 cells are adapted to grow in the animal component-free Gene Therapy Medium 3 (Sigma G9916)
- Keywords:
- Culture Type: Cell line
- Origin/Source:
- Organism:
- Species: Human (Homo sapiens)
- Age: Fetus
- Tissue of Origin: Embryonic Kidney
- Organism:
- Cell line features:
- Morphology:
- Cell Type: Epithelial
- Growth Mode: Aggregates in suspension
- Morphology:
- Applications:
- ?
- Handling and maintenance:
- Biosafety level: BSL 2 (It may differ in your country, follow the guidelines of your institute/country)
- GMO Status: GMO class 1
- Complete culture medium:
- Gene Therapy Medium 3 (Sigma G9916) + 2mM Glutamine
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO₂, 95% Air (culturecollections)
- Subculturing:
- Subculturing method: Dilution
- Split Ratio: 1:2
- Split frequency: every 4 to 5 days
- Subculturing method: Dilution
- Cryopreservation
- Freezing medium: 50:50 fresh culture medium: conditioned medium + 10% DMSO
- Cryopreservation temperature: Liquid nitrogen gas phase or -150°C freezer
- Availability:
- Culture Collections (UK Health Security Agency): Product link
- References:
- Abbreviations
- GMO (Genetically Modified Organism)
- DMSO (Dimethyl sulfoxide)
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