- Synonyms:
- 293 N3S
- HEK293 N3S
- Description:
- Original cell line: 293 (also known as HEK-293)
- Adapted for growth in suspension by passaging 293 cells in nude mice
- Keywords:
- Culture Type: Cell line
- Origin/Source:
- Organism:
- Species: Human (Homo sapiens)
- Age: Fetus
- Gender: Female
- Tissue of Origin: Embryonic Kidney
- Organism:
- Cell line features:
- Morphology:
- Cell Type: Embryonic cell
- Growth Mode: Suspension
- Cell doubling time: 30 hours
- Morphology:
- Applications:
- ?
- Handling and maintenance:
- Biosafety level: BSL 2 (It may differ in your country, follow the guidelines of your institute/country)
- GMO Status: GMO class 1
- Complete culture medium:
- Joklik’s MEM + 2mM Glutamine + 10% Horse Serum
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO₂, 95% Air
- Subculturing:
- Subculturing method: Dilution
- Maintain cultures between 5 x 104 to 5 x 105 cells/ml in a Techne stirrer
- Subculturing method: Dilution
- Cryopreservation
- Freezing medium: ?
- Cryopreservation temperature: Liquid nitrogen gas phase or -150°C freezer
- Availability:
- Culture Collections (UK Health Security Agency): Product link
- References:
- Abbreviations
- GMO (Genetically Modified Organism)
- BSL (Biosafety level)
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