Cell Line : A-204

OrganismHuman (Homo sapiens), 1 year old, female
Source/TissueMuscle, rhabdomyosarcom
Cell type 
Growth modeAdherent, monolayer
Doubling time≈ 36 hours
Life spanInfinite, continuous culture
KaryotypeNear diploid karyotype with tetraploid sideline (31%)
Drug resistance?
Biosafety Level1 REF (May differ in your country, follow the guidelines of your institute/country)
Gene Expression 
ApplicationsTransient Transfection
Related cell lines 

Cell line isolation information

The cell line A7r5 was established from the rhabdomyosarcoma of a 1-year-old girl in 1973.

Recommended propagation method

Culture medium: McCoy’s 5A + 10% FBS
Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C.
Split ratio: 1:3 to 1:6
Split frequency: Once or twice a week
Harvesting time: Subconfluent culture (80% – 90%)
Split Method: Trypsin/EDTA

Availability (Where to Get the Cell Line)

♦ American Type Culture Collection (ATCC) : Catalog No.: HTB-82
♦ Common Access to Biological Resources and Information (CABRI) : Catalog No. : ICLC HTL99002ACC 250
♦ DSMZ : Catalog No. : ACC 250
♦  CLS cell line service: Item number: 300109
♦ Accegen Biotechnology: ABC-TC445S


♦ Giard, et al., 1973. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758, Full Text –  Oxford Academic
American Type Culture Collection (ATCC)
Common Access to Biological Resources and Information (CABRI)

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