| OVERVIEW | |
| Organism | Human (Homo sapiens), 1 year old, female |
| Source/Tissue | Muscle, rhabdomyosarcom |
| Morphology | Epithelial-like |
| Cell type | |
| Growth mode | Adherent, monolayer |
| Doubling time | ≈ 36 hours |
| Life span | Infinite, continuous culture |
| Karyotype | Near diploid karyotype with tetraploid sideline (31%) |
| Transformed | |
| Tumorigenic | Yes |
| Drug resistance | ? |
| Biosafety Level | 1 REF (May differ in your country, follow the guidelines of your institute/country) |
| Gene Expression | |
| Products | ? |
| Applications | Transient Transfection |
| Remarks | |
| Related cell lines |
Cell line isolation information
The cell line A7r5 was established from the rhabdomyosarcoma of a 1-year-old girl in 1973.
Recommended propagation method
Culture medium: McCoy’s 5A + 10% FBS
Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C.
Split ratio: 1:3 to 1:6
Split frequency: Once or twice a week
Harvesting time: Subconfluent culture (80% – 90%)
Split Method: Trypsin/EDTA
Availability (Where to Get the Cell Line)
♦ American Type Culture Collection (ATCC) : Catalog No.: HTB-82
♦ Common Access to Biological Resources and Information (CABRI) : Catalog No. : ICLC HTL99002; ACC 250
♦ DSMZ : Catalog No. : ACC 250
♦ CLS cell line service: Item number: 300109
♦ Accegen Biotechnology: ABC-TC445S
REFERENCES
♦ Giard, et al., 1973. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758, Full Text – Oxford Academic
♦ American Type Culture Collection (ATCC)
♦ Common Access to Biological Resources and Information (CABRI)
♦ DSMZ
