Overview | |
| Organism | Mouse (Mus musculus) |
| Source/Tissue | Melanoma |
| Morphology | Epithelial-like |
| Cell type | |
| Growth mode | Adherent, monolayer |
| Doubling time | ≈ 20 hours |
| Life span | Infinite, continuous culture |
| Karyotype | Murine karyotype |
| Transformed | |
| Tumorigenic | |
| Drug resistance | |
| Biosafety Level | 1 REF |
| Gene Expression | |
| Products | |
| Applications | |
| Remarks | |
| Related cell lines |
Cell line isolation information
The cell line B-16V was established from a B16 melanoma tumor grown in syngeneic C57BL/6 mice.
Recommended propagation method
Culture medium: 90% RPMI 1640 + 10% FBS
Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C.
Split ratio: 1:7 to 1:10 (seeding at 1-3 x 103 cells/cm²)
Split frequency: Once or twice a week
Harvesting time: Subconfluent culture (80%)
Split Method: Trypsin/EDTA
Availability
- Common Access to Biological Resources and Information (CABRI) : Catalog No. : Product link 1; Product link 2
- DSMZ : Catalog No. : Product link
References
- Supino et al., 1986. Characterization of a doxorubicin-resistant murine melanoma line: studies on cross-resistance and its circumvention. Br J Cancer 54(1), 33-42. PMID-3730255; Full Text Link: Nature Publishing Group; PMC2001650
- Common Access to Biological Resources and Information (CABRI)
- DSMZ
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