Cell Line : B-16V

Overview

 
OrganismMouse (Mus musculus)
Source/TissueMelanoma
MorphologyEpithelial-like
Cell type 
Growth modeAdherent, monolayer
Doubling time≈ 20 hours
Life spanInfinite, continuous culture
KaryotypeMurine karyotype
Transformed 
Tumorigenic 
Drug resistance 
Biosafety Level1 REF
Gene Expression 
Products 
Applications 
Remarks 
Related cell lines 

Cell line isolation information

The cell line B-16V was established from a B16 melanoma tumor grown in syngeneic C57BL/6 mice.

Recommended propagation method

Culture medium: 90% RPMI 1640 + 10% FBS
Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C.
Split ratio: 1:7 to 1:10 (seeding at 1-3 x 103 cells/cm²)
Split frequency: Once or twice a week
Harvesting time: Subconfluent culture (80%)
Split Method: Trypsin/EDTA

Availability

References

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