Cell Line: AGS

OVERVIEW

 
OrganismHuman (Homo sapiens), 54 years caucasian female (Ref: atcc)
Source/Tissue of origin
Stomach, Gastric adenocarcinoma (Ref: atcc)
Year of isolation 
MorphologyEpithelial-like (Ref: atcc)
Cell typeGastric epithelial
Growth modeAdherent, monolayer
Doubling time
≈ 20 hrs (Ref: atcc)
Life spanInfinite, continuous culture
KaryotypeHyperdiploid (Ref: atcc)
Modal chromosome number: 49 (in 60% of cells) (Ref: atcc)
Polyploidy rate: 3.6%. (Ref: atcc)
Transformed 
TumorigenicYes (forms tumors in athymic BALB/c mice) (Ref: atcc)
Drug resistance 
Biosafety LevelBSL 2 (Ref: atcc, phe-culturecollections) (It may differ in your country, follow the guidelines of your institute/country)
Gene ExpressionDo not express E-cadherin (Oliveira et al., 2009)
Products 
Applications3D cell culture (Ref: atcc)
Suitable transfection host (Ref: atcc)
Suitable model system to study Helicobacter pylori – host interaction
Remarks 
KeywordsA human gastric epithelial adenocarcinoma cell line
Related cell lines 

Culture maintenance

Culture medium*
1. F-12K Medium + 10% fetal bovine serum (Ref: atcc)
2. Ham’s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (phe-culturecollections)
3. RPMI + 10% FBS
Culture conditionsHumidified incubator, 95% air + 5% CO2, 37°C
Subculturing procedureTrypsin-EDTA

Culture storage

Cryo-medium
Complete growth medium + 5% (v/v) (Ref: atcc)
Storage conditions
Vapor phase of liquid nitrogen (Ref: atcc)

* Several culture media can be used to maintain this cell line in culture. Use the culture medium recommended by the suppliers/source from where you have received this cell line.

REFERENCES

  • https://www.atcc.org/products/crl-1739
  • Oliveira et al., 2009. CagA associates with c-Met, E-cadherin, and p120-catenin in a multiproteic complex that suppresses Helicobacter pylori-induced cell-invasive phenotype. J Infect Dis. 200(5), 745-755. PMID-19604117; Full Text Link: academic.oup

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