OVERVIEW | |
| Organism | Human (Homo sapiens), 54 years caucasian female (Ref: atcc) |
| Source/Tissue of origin | Stomach, Gastric adenocarcinoma (Ref: atcc) |
| Year of isolation | |
| Morphology | Epithelial-like (Ref: atcc) |
| Cell type | Gastric epithelial |
| Growth mode | Adherent, monolayer |
| Doubling time | ≈ 20 hrs (Ref: atcc) |
| Life span | Infinite, continuous culture |
| Karyotype | Hyperdiploid (Ref: atcc) Modal chromosome number: 49 (in 60% of cells) (Ref: atcc) Polyploidy rate: 3.6%. (Ref: atcc) |
| Transformed | |
| Tumorigenic | Yes (forms tumors in athymic BALB/c mice) (Ref: atcc) |
| Drug resistance | |
| Biosafety Level | BSL 2 (Ref: atcc, phe-culturecollections) (It may differ in your country, follow the guidelines of your institute/country) |
| Gene Expression | Do not express E-cadherin (Oliveira et al., 2009) |
| Products | |
| Applications | 3D cell culture (Ref: atcc) Suitable transfection host (Ref: atcc) Suitable model system to study Helicobacter pylori – host interaction |
| Remarks | |
| Keywords | A human gastric epithelial adenocarcinoma cell line |
| Related cell lines | |
Culture maintenance | |
| Culture medium* | 1. F-12K Medium + 10% fetal bovine serum (Ref: atcc) 2. Ham’s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (phe-culturecollections) 3. RPMI + 10% FBS |
| Culture conditions | Humidified incubator, 95% air + 5% CO2, 37°C |
| Subculturing procedure | Trypsin-EDTA |
Culture storage | |
| Cryo-medium | Complete growth medium + 5% (v/v) (Ref: atcc) |
| Storage conditions | Vapor phase of liquid nitrogen (Ref: atcc) |
* Several culture media can be used to maintain this cell line in culture. Use the culture medium recommended by the suppliers/source from where you have received this cell line.
REFERENCES
- https://www.atcc.org/products/crl-1739
- Oliveira et al., 2009. CagA associates with c-Met, E-cadherin, and p120-catenin in a multiproteic complex that suppresses Helicobacter pylori-induced cell-invasive phenotype. J Infect Dis. 200(5), 745-755. PMID-19604117; Full Text Link: academic.oup
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