| OVERVIEW | |
| Organism | Human (Homo sapiens) |
| Source/Tissue of origin | Thyroid carcinoma |
| Morphology | Spindle- or round-shaped cells |
| Cell type | |
| Growth mode | Adherent, monolayer |
| Doubling time | ≈ 30 hours |
| Life span | Infinite, continuous culture |
| Karyotype | Near-diploid karyotype with hexaploid sideline (dsmz) |
| Transformed | |
| Tumorigenic | ? |
| Drug resistance | ? |
| Biosafety Level | 1 (dsmz) (May differ in your country, follow the guidelines of your institute/country) |
| Gene Expression | |
| Products | ? |
| Applications | |
| Remarks | |
| Related cell lines |
Cell line isolation information
The cell line B-CPAP was established from the tumor tissue of a 76- year-old woman with metastasizing papillary thyroid carcinoma in 1992.
Recommended propagation method
Culture medium: 90% RPMI + 10% FBS (heat-inactivated)
Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C.
Split ratio: 1:4 to 1:6
Split frequency: every 3-4 days
Harvesting time: Confluent culture (dsmz)
Split Method: Trypsin/EDTA
Recommended Cryopreservation Method
Freeze medium: 70% complete culture medium + 20% FBS (heat-inactivated) * + 10% DMSO
* FBS concentration can be increased upto 50%.
Storage temperature: Liquid nitrogen vapor phase
Cell density: 3 x 106 cells/ampoule
AVAILABILITY
- DSMZ : Catalog No.: Product link
- Creative-bioarray: Product link
REFERENCES
- Fabien et al., 1994. Description of a human papillary thyroid carcinoma cell line. Morphologic study and expression of tumoral markers. Cancer. 73(8), 2206-2212. PMID-8156527; Full-Text Link: onlinelibrary.wiley (Download PDF)
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