|Organism||Human (Homo sapiens)|
|Source/Tissue of origin||Thyroid carcinoma|
|Morphology||Spindle- or round-shaped cells|
|Growth mode||Adherent, monolayer|
|Doubling time||≈ 30 hours|
|Life span||Infinite, continuous culture|
|Karyotype||Near-diploid karyotype with hexaploid sideline (dsmz)|
|Biosafety Level||1 (dsmz) (May differ in your country, follow the guidelines of your institute/country)|
|Related cell lines|
Cell line isolation information
The cell line B-CPAP was established from the tumor tissue of a 76- year-old woman with metastasizing papillary thyroid carcinoma in 1992.
Recommended propagation method
Culture medium: 90% RPMI + 10% FBS (heat-inactivated)
Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C.
Split ratio: 1:4 to 1:6
Split frequency: every 3-4 days
Harvesting time: Confluent culture (dsmz)
Split Method: Trypsin/EDTA
Recommended Cryopreservation Method
Freeze medium: 70% complete culture medium + 20% FBS (heat-inactivated) * + 10% DMSO
* FBS concentration can be increased upto 50%.
Storage temperature: Liquid nitrogen vapor phase
Cell density: 3 x 106 cells/ampoule
- Fabien et al., 1994. Description of a human papillary thyroid carcinoma cell line. Morphologic study and expression of tumoral markers. Cancer. 73(8), 2206-2212. PMID-8156527; Full-Text Link: onlinelibrary.wiley (Download PDF)