Cell Line: HCC1500

  • Cell Line: HCC1500
  • Synonyms:
    • HCC-1500
    • Hamon Cancer Center 1500
  • Culture Type: Cell line
  • Keywords: Human epithelial mammary ductal carcinoma cell line
  • Description: Established from the mammary gland of a 32-year-old, Black female patient with breast invasive ductal carcinoma TMN stage IIB, grade 2.
  • Origin:
    • Organism: 
      • Species: Human (Homo sapiens)
      • Age: 32 years
      • Ethnicity: Black
      • Gender: Female
      • Tissue (origin): Breast, Mammary gland
      • Tissue (isolation): Breast, Mammary gland
      • Disease:
        • Breast Invasive Ductal Carcinoma TNM Stage IIB, grade 2
        • Primary/Metastasis: Primary
        • Molecular subtype: ERpos HER2neg (depmap)
    • Year of origin: 1995
  • Cell line features:
  • Handling and maintenance:
    • Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
    • Complete culture medium: RPMI-1640 Medium + 10% FBS
    • Culture conditions: 
      • Temperature: Humidified 37°C incubator
      • Atmosphere: 5% CO2, 95% Air
    • Subculturing:
      • Subculturing method: Enzymatic dissociation
      • Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
      • Harvesting time:
      • Split ratio: 1:2 to 1:3
      • Medium Renewal: Every 3 to 5 days
    • Cryopreservation:
      • Freezing medium:
        • Complete growth medium + 5% DMSO
      • Storage conditions: Vapor phase of liquid nitrogen
      • Cell suspension (cell density):-
      • Volume per vial:-
  • Applications: 
    • Cancer research
    • 3D cell culture
  • Availability

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