- Cell Line: HCC1806
- Synonyms:
- Hcc1806
- HCC-1806
- Hamon Cancer Center 1806
- Culture Type: Cell line
- Keywords: Human epithelial mammary acantholytic squamous cell carcinoma cell line
- Description: Established from the mammary gland of a 60-year-old, Black, female patient with acantholytic squamous cell carcinoma (ASCC), TNM Stage IIB, grade 2
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 60 years
- Ethnicity: Black
- Gender: Female
- Tissue (origin): Breast, Mammary gland
- Tissue (isolation): Breast, Mammary gland
- Disease:
- Acantholytic Squamous Cell Carcinoma; TNM Stage IIB, grade 2
- Primary/Metastasis: Primary
- Molecular subtype: ERneg HER2neg (depmap)
- Year of origin: 1995 – 1996
- Organism:
- Cell line features:
- Morphology: Epithelial-like
- Cell Type: Epithelial cell
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Differentiation status: Poorly differentiated
- Karyotype: Modal Chromosome Number = 75
- Doubling time:-
- Clinically relevant genes:
- Gene expression:
- Positive for the expression of Epithelial glycoprotein 2 (EGP2) (epithelial cell-specific marker)
- Positive for the expression of Cytokeratin 19 (epithelial cell-specific marker)
- Sequence variations (gene mutations/deletions/fusions/translocations):
- Homozygous for deletions in the FHIT gene at 3p14.2
- For information, please visit
- Morphology: Epithelial-like
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:
- Split ratio: 1:2 to 1:4
- Medium Renewal: Every 2 to 3 days
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- References
- https://www.atcc.org/products/crl-2335
- https://www.cellosaurus.org/CVCL_1258
- https://depmap.org/portal/cell_line/ACH-000624?tab=overview
- Ahmadian et al., 1997. Analysis of the FHIT gene and FRA3B region in sporadic breast cancer, preneoplastic lesions, and familial breast cancer probands. Cancer Res. 57: 3664-3668, 1997. PMID-9288768; Full Text: Silverchair (Download PDF)
- Gazdar et al., 1998. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PMID-9833771; Full Text: Wiley (Download PDF)
- Images
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- Article Authenticity Index *
- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
Was this post helpful?
Let us know if you liked the post. That’s the only way we can improve.
