- Synonyms:
- HEK-293.2sus
- Description:
- Parent cell line: 293 (also known as HEK-293)
- Progressively adapted from an anchorage-dependent growth mode to a suspension mode
- Keywords:
- Culture Type: Cell line
- Origin/Source:
- Organism:
- Species: Human (Homo sapiens)
- Age: Fetus
- Tissue of Origin: Embryonic Kidney
- Organism:
- Cell line features:
- Morphology: Rounded
- Cell Type: Embryonic cell
- Growth Mode: Suspension
- Doubling time: ≈ 34 hrs
- Karyotype: Hypotriploid, Modal chromosome number: 64
- Morphology: Rounded
- Applications:
- 3D cell culture
- Bioproduction
- Handling and maintenance
- Biosafety level: BSL 2 (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- 293 SFM II (Invitrogen, Catalog No. 11686-029) + 4 mM L-glutamine
- 293 SFM II (Invitrogen, Catalog No. 11686-029) + 4 mM L-glutamine
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO₂, 95% Air
- Subculturing:
- Subculturing procedure: Dilution
- Harvesting time: When culture reaches cell density ≈1 x 106 cells/mL
- Subcultivation Ratio: 1:6 to 1:10
- Medium Renewal: 2 to 3 times per week
- Seeding densities: 1 x 105 and 3 x 105 cells/mL
- Subculturing procedure: Dilution
- Cryopreservation
- Conditioned growth medium collected from HEK-293.2sus culture + 10% (v/v) DMSO
- Cryopreservation temperature: Liquid nitrogen gas phase or -150°C freezer
- Availability:
- American Type Culture Collection (ATCC): Product link
- References:
- Abbreviations
- DMSO (Dimethyl sulfoxide)
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