- Synonyms:
- 293[HEK-293] Cas9
- HEK 293 Cas9
- 293 Cas9
- Description:
- Parent cell line: 293 (also known as HEK-293)
- Constitutively expresses Cas9 protein
- Keywords:
- Culture Type: Cell line
- Origin/Source:
- Organism:
- Species: Human (Homo sapiens)
- Age: Fetus
- Tissue of Origin: Embryonic Kidney
- Organism:
- Cell line features:
- Morphology: Epithelial-like
- Cell Type: Epithelial
- Growth Mode: Adherent
- Morphology: Epithelial-like
- Applications:
- Suitable for CRISPR genome editing applications with high efficiency
- Handling and maintenance
- Biosafety level: BSL 2 (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- EMEM + 10% FBS
- EMEM + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO₂, 95% Air
- Subculturing:
- Cell dissociation procedure: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution
- Harvesting time: 80% confluence culture
- Subcultivation Ratio: 1:6 to 1:10
- Medium Renewal: 2 to 3 times per week
- Seeding densities: 5 x 104 – 6 x 104 viable cells/cm2
- Cell dissociation procedure: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution
- Cryopreservation
- EMEM + 7% (v/v) FBS + 5% (v/v) DMSO
- Cryopreservation temperature: Liquid nitrogen gas phase or -150°C freezer
- Availability:
- American Type Culture Collection (ATCC): Product link
- References:
- Abbreviations
- EMEM (Eagle’s Modified Eagle’s Medium)
- FBS (Fetal Bovine Serum)
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