- Synonyms:
- Culture Type: Cell line
- Keywords: hTERT-immortalized Brown Preadipocytes
- Description: The donor suffered from von Hippel-Lindau syndrome.
- Source:
- Organism:
- Species: Human (Homo sapiens)
- Age: 40 years
- Gender: Male
- Disease: von Hippel-Lindau syndrome
- Tissue: Adipose tissue
- Year of origin: 2022
- Organism:
- Cell line features:
- Morphology: Fibroblast-like
- Cell Type: Brown Preadipocytes
- Growth mode: Adherent, Monolayer
- Life span: Infinite (continuous culture)
- Karyotype: Near Diploid
- Morphology: Fibroblast-like
- Handling and maintenance:
- Biosafety level: BSL 2 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: DMEM:F12 Medium + 10% FBS + 0.5 µg/mL puromycin
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 95% Air, 5% CO2
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: Trypsin-EDTA
- Split time: Sub-confluent cultures (when cell density reaches 8.6 x 104 cell/cm2)
- Split ratio: 1:3 to 1:8
- Seeding densities: 1 x 104 and 4 x 104 viable cells/cm2
- Medium Renewal: 2 to 3 times per week
- Cryopreservation:
- Freeze medium: Complete culture medium + 8% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cells suspension (cell density): 1.0 – 1.5 x 106 viable cells/ml
- Volume per vial: 1 ml
- Freeze medium: Complete culture medium + 8% DMSO
- Applications:
- Cancer research
- Toxicology research
- Availability
- American Type Culture Collection (ATCC): Product link
- References
- Abbreviations
- BSL (Biosafety level)
- DMSO (Dimethyl sulfoxide)
- DMEM (Dulbecco’s Modified Eagle Medium)
- FBS (Fetal Bovine Serum)
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