- Cell Line: K562-s
- Synonyms
- K562-s
- K 562-s
- Culture Type: Cell line
- Keywords: Tyrosine kinase inhibitor sensitive K562 subclone
- Source
- Organism: Human (Homo sapiens), 53-year-old female
- Tissue of origin: Blood
- Tissue: Bone; Marrow, pleural effusion
- Disease: Chronic myelogenous leukemia in terminal blast crisis
- Metastatic: Pleural effusion
- Cell line features:
- Morphology: Hematopoietic
- Growth mode: Suspension
- Population doubling time: ≈ 24 to 30 hrs
- Comments
- Oncogenes: BCR-ABL of the e14a2 fusion transcript type
- Oncogenes: BCR-ABL of the e14a2 fusion transcript type
- Handling
- BSL 1 (ATCC)
(It may differ in your country, follow the guidelines of your institute/country) - Complete medium: RPMI-1640 + 10% FBS + 2 mM L-Glutamine
- Culture conditions:
- Temperature: 37°C
- Subculturing procedure: Dilution (replacement or addition of fresh culture medium)
- Maintain culture between 1 – 10 X 105 cells/mL.
- Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density). (ATCC)
- Cryopreservation
- Storage conditions Vapor phase of liquid nitrogen
- Cells per vial: Approximately 5.0 to 7.0 x 106
- Volume per vial: 1.0 mL
- BSL 1 (ATCC)
- Applications:
- The paired K562-s and K562-r clones are useful cell line models of sensitivity and resistance to a tyrosine kinase inhibitor used in the treatment of chronic myeloid leukemia.
- Availability
- ATCC: CRL-3343 ™ (ATCC)
- ATCC: CRL-3343 ™ (ATCC)
- References:
- Lozzio & Lozzio, 1975. Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome. Blood 45: 321-334, 1975. PMID-163658
- Mahon et al., 2000. Selection and characterization of BCR-ABL positive cell lines with differential sensitivity to the tyrosine kinase inhibitor STI571: diverse mechanisms of resistance. Blood 96(3): 1070-1079, PMID-10910924
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