OVERVIEW
- Culture Type: Cell line
- Synonyms: KELLY
- Keywords: Human neuroblastoma
- Source
- Organism: Human
- Source/Tissue of Origin: Brain
- Features:
- Cell Type: Neuronal
- Growth Mode: Adherent
- Cytogenetics:
- Genomic amplification of the N-myc gene
- Gene expression:
- Elevated levels of N-myc RNA and protein
- Availability
- Handling information:
- Biosafety Information: BSL 2 (Ref: culturecollections)
(It may differ in your country, follow the guidelines of your institute/country) - Complete culture medium: RPMI 1640 + 2mM Glutamine + 10% FBS
- Culture conditions:
- Temperature: 37°C
- Atmosphere: 95% Air, 5% CO2
- Biosafety Information: BSL 2 (Ref: culturecollections)
- Cell dissociation procedure: trypsin/EDTA
- Subcultivation Ratio: 1:5 to 1:20
- Seeding densities: 5000 – 20,000 cells/cm²
- Split time: Sub-confluent cultures (70-80%)
- Cell dissociation procedure: trypsin/EDTA
- References:
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