Cell Line: KELLY

OVERVIEW

• Culture Type: Cell line
• Synonyms: KELLY
• Keywords: Human neuroblastoma
• Source
  • Organism: Human 
  • Source/Tissue of Origin: Brain
• Features:
  • Cell Type: Neuronal
  • Growth Mode: Adherent
• Cytogenetics:
  • Genomic amplification of the N-myc gene
• Gene expression: 
  • Elevated levels of N-myc RNA and protein
• Availability
  • ECACC General Cell Collection, 92110411 
  • Sigma-Aldrich, 92110411 
• Handling information:
  • Biosafety Information: BSL 2 (Ref: culturecollections)
    (It may differ in your country, follow the guidelines of your institute/country)
  • Complete culture medium: RPMI 1640 + 2mM Glutamine + 10% FBS
  • Culture conditions: 
    • Temperature: 37°C
    • Atmosphere: 95% Air, 5% CO2  

• • Cell dissociation procedure: trypsin/EDTA
    • Subcultivation Ratio: 1:5 to 1:20 
    • Seeding densities: 5000 – 20,000 cells/cm²  
    • Split time: Sub-confluent cultures (70-80%)

• References: 
  • Culturecollections