- Synonyms
- K-562
- K562
- Culture Type: Cell line
- Keywords: Human chronic myeloid leukemia in blast crisis
- Source:
- Organism: Human (Homo sapiens), 53-year-old caucasian female
- Tissue of Origin: blood
- Tissue: Bone; Marrow, pleural effusion
- Disease: Chronic myeloid leukemia in blast crisis
- Cell line features:
- Morphology: round and large, single cells
- Cell Type: Lymphoblast
- Growth Mode: Suspension
- Life span: Infinite, continuous culture
- Doubling time: ≈30 – 40 hours
- Tumorigenicity: tumorigenic in nude mice
- Gene expression
- Antigen expression: CD7 (25%)
- Cytogenetics:
- Human hypotriploid karyotype
- Carry Philadelphia chromosome with a BCR-ABL fusion gene
- Handling
- Biosafety Information: BSL 2 (ECACC)
(It may differ in your country, follow the guidelines of your institute/country) - Complete culture medium:
- RPMI 1640 (2mM Glutamine) + 10% FBS
- Culture conditions
- Temperature: 37°C
- Atmosphere: 95% Air, 5% CO2
- Subculturing procedure: Dilution (replacement or addition of fresh culture medium)
- Maintain cultures between 1 x 105 viable cells/mL to 1 x 106 cells/mL
- Medium Renewal: Every 2 to 3 days
- Split ratio: 1:3 to 1:5 every 3 days
- Cryopreservation
- Storage conditions: Vapor phase of liquid nitrogen
- Freezing medium: Complete growth medium + 5% (v/v) DMSO (ATCC)
- Biosafety Information: BSL 2 (ECACC)
- Applications:
- 3D cell culture
- Immune system disorder research
- Immunology
- Suitable as a transfection host
- Highly sensitive in vitro target for the natural killer assay to assess human natural killer activity
- References
- ATCC
- European Collection of Authenticated Cell Cultures (ECACC)
- Koeffler & Golde, 1980. Human myeloid leukemia cell lines: a review. Blood 56: 344-350, 1980. PMID-6996765
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