Cell Line: MCF7


OrganismHuman (Homo sapiens), 54 years caucasian female (Ref: atcc)
Source/Tissue of origin
Stomach, Gastric adenocarcinoma (Ref: atcc)
Year of isolation 
MorphologyEpithelial-like (Ref: atcc)
Cell typeGastric epithelial
Growth modeAdherent, monolayer
Doubling time
≈ 20 hrs (Ref: atcc)
Life spanInfinite, continuous culture
KaryotypeHyperdiploid (Ref: atcc)
Modal chromosome number: 49 (in 60% of cells) (Ref: atcc)
Polyploidy rate: 3.6%. (Ref: atcc)
TransformedYes, in nude mice (estrogen-dependent) (Ref: Osborne et al., 1985)
Drug resistance 
Biosafety LevelBSL 1 (Ref: atcc) (It may differ in your country, follow the guidelines of your institute/country)
Gene Expression 
Applications3D cell culture (Ref: atcc)
Suitable transfection host (Ref: atcc)
KeywordsA human mammary epithelial adenocarcinoma cell line
Related cell lines 

Culture maintenance

Culture medium*
DMEM + 10% FBS
Culture conditionsHumidified incubator, 95% air + 5% CO2, 37°C
Subculturing procedureTrypsin-EDTA

Culture storage

Complete growth medium + 5% (v/v) (Ref: atcc)
Storage conditions
Vapor phase of liquid nitrogen (Ref: atcc)


  • https://www.atcc.org/products/crl-1739
  • Sutherland et al., 1983. Cell proliferation kinetics of MCF-7 human mammary carcinoma cells in culture and effects of tamoxifen on exponentially growing and plateau-phase cells. Cancer Res. 43(9), 3998-4006. PMID-6871841
  • Osborne et al., 1985. Effect of estrogens and antiestrogens on growth of human breast cancer cells in athymic nude mice. Cancer Res. 45(2), 584-90. PMID-3967234; Full-Text Links: Silverchair

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