Cell Line: MDA-MB-231

OrganismHuman (Homo sapiens), 51 years old white female (Ref: ATCC)
Source/Tissue of originBreast, Mammary gland (Ref: ATCC)
Established from a pleural effusion of metastatic breast adenocarcinoma (Ref: ATCC)
Year of isolation 
MorphologyEpithelial-like (Ref: ATCC)
Cell typeEpithelial (Ref: ATCC)
Growth modeAdherent (Ref: ATCC)
Doubling time?
Life spanInfinite, continuous culture
Karyotypenear-triploid (Ref: ATCC)
TransformedNaturally transformed (can form colonies in semisolid medium)
TumorigenicYes (in nude mice) (Ref: ATCC)
Drug resistance?
Gene Expression
Positive for 
Altered gene 
Applications3D cell culture (Ref: ATCC)
Suitable transfection host (Ref: ATCC)
KeywordsHuman breast adenocarcinoma
Related cell lines 
Culture maintenance
Biosafety LevelBSL 1 (Ref: ATCC)
(It may differ in your country, follow the guidelines of your institute/country)
Culture medium*Leibovitz’s L-15 medium (+ 2mM Glutamine) + 10% FBS (Ref: ATCC
Culture conditionsHumidified incubator, 37°C
CO2 is not required
Subculturing procedureTrypsin-EDTA
Culture storage
Cryo-mediumComplete growth medium + 5% (v/v) (Ref: ATCC
Storage conditionsVapor phase of liquid nitrogen (Ref: ATCC


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