- Cell Line: NCI-H2087
- Synonyms:
- NCI-H2087
- NCIH2087
- H2087
- H-2087
- Culture Type: Cell line
- Keywords: Human Lung Adenocarcinoma Cell Line
- Description: Established from lymph node metastasis of a 69-year-old, Caucasian, male patient with non-small cell lung cancer, stage 1 (lung adenocarcinoma)
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 69 years
- Ethnicity: Caucasian
- Gender: Male
- Tissue (origin): Lung
- Tissue (isolation): Lymph node (metastatic tumor)
- Disease:
- Non-Small Cell Lung Cancer, Stage 1
- Disease subtype: Lung Adenocarcinoma
- Molecular subtype: NSCLC Adenocarcinoma
- Primary/Metastasis: Metastatic
- Non-Small Cell Lung Cancer, Stage 1
- Year of origin: 1988
- Organism:
- Cell line features:
- Cell Type:
- Morphology: Epithelial-like and/or rounded
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time: 51 hours
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Tumorigenicity:-
- Gene expression:-
- Sequence variations:
- NRAS (Q61K mutation, Oncogenic, Gain-of-function, Heterozygous)
- TP53 (V157F mutation, Likely Oncogenic, Likely Loss-of-function, Homozygous)
- BRAF (L597V mutation, Oncogenic, Gain-of-function, Heterozygous)
- PAK5 (T538N mutation, Likely Oncogenic, Likely Gain-of-function)
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- Cell Type:
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- RPMI-1640 Medium + 5% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:-
- Split ratio: 1:2 to 1:4
- Medium Renewal: twice weekly
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium:
- RPMI 1640 medium + 10% (v/v) FBS + 5% (v/v) DMSO
- RPMI 1640 medium + 10% (v/v) FBS + 5% (v/v) DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- References
- Images:–
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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