- Cell Line: NCI-H727
- Synonyms:
- NCI-H727
- NCIH727
- H727
- H-727
- Culture Type: Cell line
- Keywords: Human Lung Carcinoid Epithelial Cell line
- Description: Established from the lungs of a 65-year-old, White female with a carcinoid tumor
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 65 years
- Ethnicity: White
- Gender: Female
- Tissue (origin): Lung
- Disease:
- Lung Neuroendocrine Tumor
- Disease subtype: Lung Carcinoid
- Primary/Metastasis: Primary
- Lung Neuroendocrine Tumor
- Year of origin:-
- Organism:
- Cell line features:
- Cell Type: Epithelial
- Morphology: Epithelial-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time: 41 hours
- Microsatellite instability: Stable
- Special features: The best differentiated available bronchial carcinoid cell line
- Product:
- Cells synthesize Peptide neuromedin B (NMB)
- Cells do not synthesize gastrin-releasing peptide (GRP)
- Tumorigenicity:-
- Gene expression:
- Cells can synthesize Epidermal growth factor (EGF)
- Cells express easily detectable levels of p53 mRNA compared to levels found in normal lung
- Sequence variations:
- KRAS (G12V mutation, Oncogenic, Gain-of-function, Heterozygous)
- For information, please visit
- Cell Type: Epithelial
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time: Sub-confluent cultures (70-80%)
- Seeding density: 2 – 4 x104 cells/cm2
- Split ratio: 1:3 to 1:6
- Medium Renewal: Every 2 to 3 days
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% DMSO (ATCC)
- Culture medium + 50% FBS + 10% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Availability:
- American Type Culture Collection (ATCC): Product link
- UK Health Security Agency Culture Collections: Product link
- Sigma Aldrich: Product link
- References
- Images:
- NCI-H727 cell image link (Source: culturecollections)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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