Cell Line: NCI-H727

  • Cell Line: NCI-H727
  • Synonyms:
    • NCI-H727
    • NCIH727
    • H727
    • H-727
  • Culture Type: Cell line
  • Keywords: Human Lung Carcinoid Epithelial Cell line 
  • Description: Established from the lungs of a 65-year-old, White female with a carcinoid tumor
  • Origin:
    • Organism: 
      • Species: Human (Homo sapiens)
      • Age: 65 years
      • Ethnicity: White
      • Gender: Female
    • Tissue (origin): Lung
    • Disease:
      • Lung Neuroendocrine Tumor
        • Disease subtype: Lung Carcinoid
      • Primary/Metastasis: Primary
    • Year of origin:-
  • Cell line features:
  • Handling and maintenance:
    • Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
    • Complete culture medium: RPMI-1640 Medium + 10% FBS
    • Culture conditions: 
      • Temperature: Humidified 37°C incubator
      • Atmosphere: 5% CO2, 95% Air
    • Subculturing:
      • Subculturing method: Enzymatic dissociation
      • Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
      • Harvesting time: Sub-confluent cultures (70-80%)
      • Seeding density: 2 – 4 x104 cells/cm2
      • Split ratio: 1:3 to 1:6
      • Medium Renewal: Every 2 to 3 days
    • Cryopreservation:
      • Freezing medium:
        • Complete growth medium + 5% DMSO (ATCC)
        • Culture medium + 50% FBS + 10% DMSO
      • Storage conditions: Vapor phase of liquid nitrogen
      • Cell suspension (cell density):-
      • Volume per vial:
  • Applications: 
    • Cancer research
    • 3D cell culture

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