- Cell Line: PANC-1
- Synonyms:
- Panc1
- Panc-1
- Panc 1
- PANC.1
- PanC1
- PANC1
- Culture Type: Cell line
- Keywords: Human Pancreatic Adenocarcinoma Epithelial Cell Line
- Description: Established from a primary tumor taken from the pancreas of a 56-year-old Caucasian male patient with pancreatic adenocarcinoma
- Origin:
- Cell line features:
- Cell Type: Epithelial cell
- Morphology: Epithelial-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype: hypertriploid
- Doubling time: ≈ 52 h
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- KRAS (G12D mutation, Oncogenic, Gain-of-function)
- TP53 (R273H mutation, Oncogenic, Loss-of-function)
- For more information, please visit
- KRAS (G12D mutation, Oncogenic, Gain-of-function)
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: DMEM + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time: Sub-confluent culture
- Split ratio: 1:2 to 1:4
- Medium Renewal: Every 2 to 3 days.
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Freezing medium: Complete growth medium + 5% DMSO
- Applications:
- Cancer research
- 3D cell culture
- Cancer research
- Availability:
- American Type Culture Collection (ATCC): Product link
- UK Health Security Agency Culture Collections: Product link
- References:
- ATCC
- Cellosaurus
- depmap
- Lieber et al., 1975. Establishment of a continuous tumor-cell line (panc-1) from a human carcinoma of the exocrine pancreas. Int. J. Cancer 15: 741-747. PMID-1140870
- Images:
- PANC-1 cell image (Source: ATCC)
- PANC-1 cell image (Source: depmap)
- PANC-1_24hr post seeding (Source: culturecollections)
- PANC-1_48hr post seeding (Source: culturecollections)
- PANC-1_72hr post seeding (Source: culturecollections)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- DMEM (Dulbecco’s Modified Eagle’s Medium)
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