- Synonyms:
- Piezo1 Knock-out HEK293T
- Description:
- Original cell line: 293 (also known as HEK-293)
- Immediate parental line: HEK293T
- Piezo1 knock-out HEK293T (no expression of Piezo1)
- Keywords:
- Culture Type: Cell line
- Origin/Source:
- Organism:
- Species: Human (Homo sapiens)
- Age: Fetus
- Tissue of Origin: Embryonic Kidney
- Organism:
- Cell line features:
- Morphology: Epithelial-like
- Cell Type: Epithelial
- Growth Mode: Adherent
- Morphology: Epithelial-like
- Applications:
- Can be used to assess ion channel activity by electrophysiology without any interference from endogenous mechanically activated currents caused by the ion channel Piezo1
- Can be used to assess ion channel activity by electrophysiology without any interference from endogenous mechanically activated currents caused by the ion channel Piezo1
- Handling and maintenance:
- Biosafety level: BSL 2 (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- DMEM (2mM L-glutamine) + 10% FBS
- DMEM (2mM L-glutamine) + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO₂, 95% Air
- Subculturing:
- Cell dissociation procedure: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution
- Harvesting time: 80% confluence culture
- Subcultivation Ratio: 1:3 to 1:8
- Medium Renewal: 2 to 3 times per week
- Seeding densities: 1 x 104 viable cells/cm2
- Cell dissociation procedure: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution
- Cryopreservation
- Freezing medium: DMEM (2 mM L-Glutamine) + 10% (v/v) Heat-Inactivated FBS + 5% (v/v) DMSO
- Cryopreservation temperature: Liquid nitrogen gas phase or -150°C freezer
- Cells per vial: Approximately 2.0 to 3.0 x 106
- Volume per vial: 1.0 mL
- Availability:
- American Type Culture Collection (ATCC): Product link
- References:
- https://www.atcc.org/products/crl-3519
- Lukacs et al., 2015. Impaired PIEZO1 function in patients with a novel autosomal recessive congenital lymphatic dysplasia. Nat Commun 6:8329. PMID-26387913; Full-Text Links: Nature, PMC
- Abbreviations
- DMEM (Dulbecco’s Modified Eagle’s Medium)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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