- Synonyms:
- 293T/17 SF
- HEK 293T/17 SF
- Description:
- Original cell line: 293 (also known as HEK-293)
- Parent cell line: 293T/17 (293 cells carrying a temperature-sensitive SV40 T-antigen mutant tsA1609, Clone 17)
- Derivative of 293T/17 cell line, adapted serum-free medium and suspension mode (anchorage-independent growth mode)
- Keywords:
- Culture Type: Cell line
- Origin/Source:
- Organism:
- Species: Human (Homo sapiens)
- Age: Fetus
- Tissue of Origin: Embryonic Kidney
- Organism:
- Cell line features:
- Morphology: Lymphocyte-like (single cells to small aggregates)
- Cell Type: Embryonic cell
- Growth Mode: Suspension
- Doubling time: ≈ 24 hrs
- Morphology: Lymphocyte-like (single cells to small aggregates)
- Applications:
- 3D cell culture
- Optimal for transient transfection and protein expression
- Handling and maintenance:
- Biosafety level: BSL 2 (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- BalanCD HEK293 (Irvine Scientific # 91165) + 8 mM L-glutamine + 10 µL/mL Insulin-Transferrin-Selenium (ITS) (Corning # 25-800-CR)
- BalanCD HEK293 (Irvine Scientific # 91165) + 8 mM L-glutamine + 10 µL/mL Insulin-Transferrin-Selenium (ITS) (Corning # 25-800-CR)
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO₂, 95% Air
- Subculturing:
- Subculturing procedure: Dilution
- Harvesting time: When culture reaches cell density ≈2 x 106 cells/mL
- Subculturing frequency: Every 2nd or 3rd day
- Subcultivation Ratio:
- Medium Renewal:
- Seeding densities: 4 x 105 and 5 x 105 cells/mL
- Subculturing procedure: Dilution
- Cryopreservation
- 2X freezing medium: Complete Growth Medium supplemented with 15% DMSO (prepare cell suspension in conditioned medium. Add 1:1 Cell suspension: 2x freezing medium ratio)
- Cryopreservation temperature: Liquid nitrogen gas phase or -150°C freezer
- Cells per vial: 5-7 x 106 cells/mL
- Volume per vial: 1 mL
- 2X freezing medium: Complete Growth Medium supplemented with 15% DMSO (prepare cell suspension in conditioned medium. Add 1:1 Cell suspension: 2x freezing medium ratio)
- Availability:
- American Type Culture Collection (ATCC): Product link
- References:
- Abbreviations
- DMSO (Dimethyl sulfoxide)
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