- Cell Line: THP-1
- Synonyms:
- THP1
- THP 1
- THP-1
- Culture Type: Cell line
- Keywords: Human acute monocytic leukemia cell line
- Description: Established from the peripheral blood of a 1-year-old boy with acute monocytic leukemia (AML) at relapse
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 1 year
- Ethnicity:-
- Gender: Male
- Tissue: Peripheral blood
- Disease:
- Acantholytic Acute monocytic leukemia
- Molecular subtype: M5 (depmap)
- Acantholytic Acute monocytic leukemia
- Year of origin: 1978
- Organism:
- Cell line features:
- Cell Type: Monocyte
- Morphology: large, round
- Growth mode: single cells in suspension, partly in clusters
- Life span: Infinite (continuous culture)
- Differentiation: differentiation can be induced with the phorbol ester TPA
- Karyotype: Near-tetraploid
- Doubling time: ≈26 h
- Gene expression:
- Cells express Complement (C3)
- Cells express Fc
- Cells produce lysozyme and show phagocytic activity
- Cells express Complement (C3)
- Sequence variations (gene mutations/deletions/fusions/translocations):
- Cells carry t(9;11)(p21;q23) leading to MLL-AF9 fusion gene
- NRAS (G12D mutation, Oncogenic, Gain-of-function)
- For information, please visit
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 0.05 mM 2-mercaptoethanol + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Maintain culture between 0.2 x 106 cells/ml to 1.0 x 106 cells/ml
- Harvesting time: When culture density reaches 8 x105 cells/mL. Do not allow the cell density to exceed 1 x 106 cells/mL. (ATCC)
- Split ratio: 1:2 to 1:3
- Medium Renewal: Every 2 to 3 days
- Subculturing method: Dilution
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% DMSO (ATCC)
- Complete growth medium + 20% FBS + 10% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density): 3 – 4 x 106 cells/ml
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Immunology
- Immuno-oncology
- Toxicology
- Cell and gene therapy (CGT) development
- Availability
- American Type Culture Collection (ATCC): Product link
- References
- ATCC
- Cellosaurus
- Depmap
- Tsuchiya et al., 1980. Establishment and characterization of a human acute monocytic leukemia cell line (THP-1). Int J Cancer. 26(2), 171-176. PMID-6970727; Full Text: Wiley
- Tsuchiya et al., 1982. Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester. Cancer Res. 42: 1530-1536. PMID-6949641; Full Text: Silverchair (Download PDF)
- Skubitz et al., 1983. Human granulocyte surface molecules identified by murine monoclonal antibodies. J. Immunol. 131, 1882-1888. PMID-6619543; Full Text: aai, Archive (Download PDF)
- Images
- THP-1 cell image (Source: ATCC)
- THP-1 cell image (Source: depmap)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- TPA (12-O-tetradecanoylphorbol-13-acetate)
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- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
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