Cell Line : U3082MG

CELL LINE ISOLATION INFORMATION

The cell line U3082MG was established from a Glioblastoma tumor biopsy taken from a 70 years old female patient.  

Dissociated cells from tumor tissue was allowed to grow as spheres onto uncoated dishes in complete culture medium [1:1 mix of Neurobasal and DMEM/F12 media supplemented with N2 (1x final conc),  B27 (1x final conc), human recombinant FGF2 (final conc. 10 ng/ml) and human EGF (final conc. 10 ng/ml)]. These neurospheres were plated onto laminin coated dishes after 5 to 7 days to allow them to grow as adherent culture. Please read Xie et al., 2015 supplementary data for exact details.

Recommended propagation method

Culture medium: Neurobasal and DMEM/F12 media (1:1 mix) containing N2 and B27 supplements and human recombinant FGF2 and EGF (10 ng/ml)
Culture dishes: Primaria dishes coated with mouse laminin 
Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C
Split ratio: 1:4 to 1:6
Split frequency: ?
Harvesting time: Subconfluent culture
Split Method: TrypLE

Recommended Cryopreservation Method

Freeze medium: Culture medium (complete medium containing all ingredients including FGF2 and EGF) supplemented with 10% DMSO
Storage temperature: Liquid nitrogen vapor phase / -150 °C freezer
Cell density: ≈ 5 x 10⁵ cells/ml

Availability

• HGCC Biobank

REFERENCES

• Xie et al., 2015. The Human Glioblastoma Cell Culture Resource: Validated Cell Models Representing All Molecular Subtypes. EBioMedicine. 2015 Aug 15;2(10):1351-63. PMID-26629530; Full Text Link: thelancet (open access), PMC