Note: The following study has analyzed Hypoxia-Induced metastasis of tumor cells in zebrafish. For more details and accuracy, please read the recommended references carefully.
- Lee and coworkers implanted T241 fibrosarcoma tumor cells into the perivitelline cavity of 48 h post-fertilized fli1:EGFP zebrafish embryos and studied the invasion of tumor cells under hypoxic conditions. They observed significantly more disseminated tumor cells away from primary implantation sites under hypoxic conditions compared to normoxic conditions.
- Animal: fli1:EGFP zebrafish
- Cell line: DiI-labeled T241 fibrosarcoma tumor cell line
- Lee and coworkers implanted LLC tumor cells into the perivitelline cavity of 48 h post-fertilized fli1:EGFP zebrafish embryos and studied the invasion of tumor cells under hypoxic conditions. They observed significantly more disseminated tumor cells away from primary implantation sites under hypoxic conditions compared to normoxic conditions.
- Animal: fli1:EGFP zebrafish
- Cell line: DiI-labeled LLC tumor cell line
ABBREVIATIONS
- DiI: 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate
- EGFP: Enhanced Green Fluorescent Protein
- fli1:EGFP: Zebrafish expressing EGFP under the fli1 promoter. In these zebrafishes, all blood vessels are labeled with EGFP
- LLC: Lewis Lung Carcinoma
REFERENCES
Lee et al., 2009. Hypoxia-induced pathological angiogenesis mediates tumor cell dissemination, invasion, and metastasis in a zebrafish tumor model. Proc Natl Acad Sci U S A. 106 (46), 19485-19490. PMID-19887629; Full-Text Links: PNAS, PMC
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