| OVERVIEW | |
| Organism | Human (Homo sapiens), 36 years, White, Female |
| Source/Tissue of origin | Peripheral blood, Acute promyelocytic leukemia |
| Morphology | Lymphoblast-like |
| Cell type | |
| Growth mode | Suspension |
| Doubling time | |
| Life span | Infinite, continuous culture |
| Karyotype | |
| Transformed | |
| Tumorigenic | Tumorigenic in nude mice |
| Drug resistance | |
| Biosafety Level | 2 (atcc) (May differ in your country, follow the guidelines of your institute/country) |
| Gene Expression | |
| Products | |
| Applications | |
| Remarks | Subclone of HL-60 cell line |
| Related cell lines |
Cell line isolation information
The cell line HL-60/S4 is a subclone of the HL-60 cell line. HL-60/S4 cells have the ability to differentiate into granulocytes, macrophages, and monocytes more quickly than the parent HL-60 cells.
Recommended propagation method
Culture medium: RPMI 1640 + 2 mM Glutamine + 10% FBSS
Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C.
Split ratio: 1:2 to 1:4
Split frequency:
Harvesting time: when the cell density is 2 x 106 cells/ml
Split Method: Dilution
Seeding density: 1 – 2 x 105 cells/ml
Recommended Cryopreservation Method
Freeze medium:
Complete culture medium + 20% FBS * + 10% DMSO (* One can increase the percentage of FBS upto 50%)
Complete growth medium supplemented with 5% (v/v) DMSO (atcc)
Storage temperature: Liquid nitrogen vapor phase
Cell density:
AVAILABILITY
- ATCC : Product link
REFERENCES
- Welch et al., 2017. Transcriptomes reflect the phenotypes of undifferentiated, granulocyte and macrophage forms of HL-60/S4 cells. Nucleus. 8(2), 222-237. PMID-28152343; Full-Text Links: tandfonline (download PDF), PMC5403136 (download PDF)
- Olins et al., 2014. Retrotransposon Alu is enriched in the epichromatin of HL-60 cells. Nucleus. 5(3), 237-246. PMID-24824428; Full-Text Links: tandfonline (download PDF), PMC4133219 (download PDF)
- Olins et al., 1998. Retinoic acid induction of nuclear envelope-limited chromatin sheets in HL-60. Exp Cell Res. 245(1), 91-104. PMID-9828104; Full-Text Links: sciencedirect
- Leung et al., 1992. Changes in microtubules, microtubule-associated proteins, and intermediate filaments during the differentiation of HL-60 leukemia cells. Cancer Res. 52(4), 949-954. PMID-1737356; Full-Text Links: aacrjournals (download PDF)
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