OrganismHuman (Homo sapiens), 36 years, White, Female
Source/Tissue of origin
Peripheral blood, Acute promyelocytic leukemia
Cell type 
Growth modeSuspension
Doubling time
Life spanInfinite, continuous culture
Tumorigenic Tumorigenic in nude mice
Drug resistance 
Biosafety Level2 (atcc) (May differ in your country, follow the guidelines of your institute/country)
Gene Expression 
RemarksSubclone of HL-60 cell line
Related cell lines 

Cell line isolation information

The cell line HL-60/S4 is a subclone of the HL-60 cell line. HL-60/S4 cells have the ability to differentiate into granulocytes, macrophages, and monocytes more quickly than the parent HL-60 cells.

Recommended propagation method

Culture medium: RPMI 1640 + 2 mM Glutamine + 10% FBSS
Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C.
Split ratio: 1:2 to 1:4
Split frequency:
Harvesting time: when the cell density is 2 x 106 cells/ml
Split Method: Dilution
Seeding density: 1 – 2 x 105 cells/ml

Recommended Cryopreservation Method

Freeze medium:
Complete culture medium + 20% FBS *  + 10% DMSO (* One can increase the percentage of FBS upto 50%)
Complete growth medium supplemented with 5% (v/v) DMSO (atcc)

Storage temperature: Liquid nitrogen vapor phase

Cell density:



  • Welch et al., 2017.  Transcriptomes reflect the phenotypes of undifferentiated, granulocyte and macrophage forms of HL-60/S4 cells. Nucleus. 8(2), 222-237. PMID-28152343; Full-Text Links: tandfonline (download PDF), PMC5403136 (download PDF)
  • Olins et al., 2014. Retrotransposon Alu is enriched in the epichromatin of HL-60 cells. Nucleus. 5(3), 237-246. PMID-24824428; Full-Text Links: tandfonline (download PDF), PMC4133219 (download PDF)
  • Olins et al., 1998. Retinoic acid induction of nuclear envelope-limited chromatin sheets in HL-60. Exp Cell Res. 245(1), 91-104. PMID-9828104; Full-Text Links: sciencedirect
  • Leung et al., 1992. Changes in microtubules, microtubule-associated proteins, and intermediate filaments during the differentiation of HL-60 leukemia cells. Cancer Res. 52(4), 949-954. PMID-1737356; Full-Text Links: aacrjournals (download PDF)

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