TBE (Tris-Borate-EDTA) Electrophoresis Buffer Calculator

The TBE (Tris-Borate-EDTA) electrophoresis buffer contains Tris base, Boric acid, EDTA and has pH 8.3. Usually the stock TBE electrophoresis buffer is prepared as a 5x or 10x concentrated solution which is diluted to 1x or 0.5x working solution.

A 1x TBE electrophoresis buffer contains 89 mM Tris base, 89 mM Boric acid and 2 mM EDTA.

Select TBE buffer concentration
Tris Base
Boric acid
Select EDTA Type *
* EDTA is available in several forms including anhydrous and EDTA disodium dihydrate form. Both forms can be used for the preparation of a TBE buffer. A 0.5M EDTA stock solution can also be used for the preparation of a TBE buffer. Remember that EDTA form has an effect on heating of electrophoresis during electrophoresis with EDTA stock solution (already dissolved, you just need to mix) causing highest heating effect and least heating with anhydrous EDTA (most difficult to dissolve).
Fill the desired volume in the box

(Change the value)

To prepare 1000 ml of 1 x TBE buffer, weigh out

10.71 g Tris base (C4H11NO3, Molecular Weight: 121.14),

5.46 g Boric acid (H3BO3, Molecular Weight: 61.83) and

0.58 g of Anhydrous EDTA

and dissolve them in deionized/distilled water to a final volume 1000 ml

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