Preparation of 0.5 M EDTA Solution from Disodium EthyleneDiamineTetraacetate Dihydrate (EDTA.Na2.2H2O)
EDTA’s ability to sequester divalent cations from solution without any precipitation makes it a suitable choice to stop an ongoing enzymatic reaction or protect DNA from degradation. Disodium salt of EDTA (EDTA.Na2.2H2O) is most commonly used to prepare 0.5 M EDTA stock solution. A 0.5 M EDTA solution can be prepared by dissolving 186.12 grams of EDTA.Na2.2H2O (Molecular Weight: 372.24) in water to a final volume of 1000 ml.
Protocol – Passaging/Subculturing Suspension Culture
Suspension culture is passaged by diluting the existing culture. Since cells float in the medium in suspension culture, they are not treated with a trypsin-EDTA solution. To subculture a suspension cell line, a small amount of cell suspension from the existing culture is transferred to a culture dish containing fresh growth medium.