Preparation of Stock Solution of Streptomycin (50 mg/ml)

Overview

  • Streptomycin function by inhibiting protein synthesis in bacteria.
  • Binding of streptomycin to the small 16S rRNA of the 30S subunit of the bacterial ribosome subunit prevents access of aminoacyl tRNA to the acceptor site on the mRNA – ribosome complex, leading to inhibition of protein synthesis.
  • It binds to the small 16S rRNA of the 30S subunit of the bacterial ribosome, interfering with the binding of formylmethionyl-tRNA to the 30S subunit.

Requirement

Reagents
> Streptomycin
> Deionized / Milli-Q water

Equipment and disposables
> Measuring cylinder
> Conical flask / Beaker / 15 ml screw-capped tube
> Magnetic stirrer

Composition
50 mg/ml streptomycin

Objective
Preparation of 10 ml of 50 mg/ml streptomycin stock solution in water

Preparation

Step 1: To prepare 10 ml of stock solution of streptomycin, weigh out 100 mg of streptomycin. Transfer it to a 15 ml screw-capped tube/beaker/conical flask. Add 9 ml of deionized / Milli-Q water. Mix until all streptomycin dissolves completely.

Tip:
We recommend using a 15 ml screw-capped tube with milliliter marks. Transfer all the contents in it. Add 9 ml deionized / Milli-Q water and mix the content by rotating the tube.

Precautions:
1. Do not dissolve in 10 ml of deionized / Milli-Q water. It is always a good practice to dissolve all ingredients first and then adjust the volume to the desired volume with the solvent. 
2. Use autoclaved deionized / Milli-Q water.

Step 2: Adjust the volume to 10 ml with deionized / Milli-Q water. Mix it again.

Storage
Store the solution in small aliquots (1 ml) at -20°C for a long time.

Note:
It is recommended to store the stock solution in small aliquots.

Application
A selection marker for bacterial culture during gene cloning experiments.

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