Overview
- Streptomycin function by inhibiting protein synthesis in bacteria.
- Binding of streptomycin to the small 16S rRNA of the 30S subunit of the bacterial ribosome subunit prevents access of aminoacyl tRNA to the acceptor site on the mRNA – ribosome complex, leading to inhibition of protein synthesis.
- It binds to the small 16S rRNA of the 30S subunit of the bacterial ribosome, interfering with the binding of formylmethionyl-tRNA to the 30S subunit.
Requirement
Reagents
> Streptomycin
> Deionized / Milli-Q water
Equipment and disposables
> Measuring cylinder
> Conical flask / Beaker / 15 ml screw-capped tube
> Magnetic stirrer
Composition
50 mg/ml streptomycin
Objective
Preparation of 10 ml of 50 mg/ml streptomycin stock solution in water
Preparation
Step 1: To prepare 10 ml of stock solution of streptomycin, weigh out 500 mg of streptomycin. Transfer it to a 15 ml screw-capped tube/beaker/conical flask. Add 9 ml of deionized / Milli-Q water. Mix until all streptomycin dissolves completely.
Tip:
We recommend using a 15 ml screw-capped tube with milliliter marks. Transfer all the contents in it. Add 9 ml deionized / Milli-Q water and mix the content by rotating the tube.
Precautions:
1. Do not dissolve in 10 ml of deionized / Milli-Q water. It is always a good practice to dissolve all ingredients first and then adjust the volume to the desired volume with the solvent.
2. Use autoclaved deionized / Milli-Q water.
Step 2: Adjust the volume to 10 ml with deionized / Milli-Q water. Mix it again.
Storage
Store the solution in small aliquots (1 ml) at -20°C for a long time.
Note:
It is recommended to store the stock solution in small aliquots.
Application
A selection marker for bacterial culture during gene cloning experiments.
