Preparation of Stock Solution of Kanamycin (50 mg/ml)


  • Kanamycin is a water-soluble, aminoglycoside bactericidal antibiotic.
  • It was originally purified from the bacterium Streptomyces kanamyceticus.
  • It inhibits protein synthesis in prokaryotes by binding to the 30S subunit of the bacterial ribosomes.
  • Kanamycin is effective against a wide variety of gram-negative and some gram-positive bacteria.


> Kanamycin
> Deionized / Milli-Q water

Equipment and disposables
> Measuring cylinder

> Conical flask / Beaker / 15 ml screw-capped tube
> Magnetic stirrer

50 mg/ml kanamycin

Preparation of 10 ml of 50 mg/ml kanamycin stock solution in water


Step 1: To prepare 10 ml of stock solution of kanamycin, weigh out 500 mg of kanamycin. Transfer it to a 15 ml screw-capped tube. Add 9 ml of deionized / Milli-Q water. Mix until all kanamycin is completely dissolved.

We recommend using a 15 ml screw-capped tube with milliliter marks. Transfer all the contents in it. Add 9 ml deionized / Milli-Q water and mix the content by rotating the tube.

1. Do not dissolve in 10 ml of deionized / Milli-Q water. It is always a good practice to dissolve all ingredients first and then adjust the volume to the desired volume with the solvent. 
2. Use autoclaved deionized / Milli-Q water.

Step 2: Adjust the volume to 10 ml with deionized / Milli-Q water. Mix it again.

Store the solution in small aliquots (1 ml) at -20°C for a long time.

A selection marker for bacterial culture in gene cloning experiments.

Follow the table to prepare a kanamycin solution of different concentrations and volumes.
Conc. / Volume5 ml10 ml25 ml100 ml
50 mg / ml0.25 g0.5 g1.25 g5 g
100 mg / ml0.5 g1 g2.5 g10 g

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