Overview
- Kanamycin is a water-soluble, aminoglycoside bactericidal antibiotic.
- It was originally purified from the bacterium Streptomyces kanamyceticus.
- It inhibits protein synthesis in prokaryotes by binding to the 30S subunit of the bacterial ribosomes.
- Kanamycin is effective against a wide variety of gram-negative and some gram-positive bacteria.
Requirement
Reagents
> Kanamycin
> Deionized / Milli-Q water
Equipment and disposables
> Measuring cylinder
> Conical flask / Beaker / 15 ml screw-capped tube
> Magnetic stirrer
Composition
50 mg/ml kanamycin
Objective
Preparation of 10 ml of 50 mg/ml kanamycin stock solution in water
Preparation
Step 1: To prepare 10 ml of stock solution of kanamycin, weigh out 500 mg of kanamycin. Transfer it to a 15 ml screw-capped tube. Add 9 ml of deionized / Milli-Q water. Mix until all kanamycin is completely dissolved.
Tip:
We recommend using a 15 ml screw-capped tube with milliliter marks. Transfer all the contents in it. Add 9 ml deionized / Milli-Q water and mix the content by rotating the tube.
Precautions:
1. Do not dissolve in 10 ml of deionized / Milli-Q water. It is always a good practice to dissolve all ingredients first and then adjust the volume to the desired volume with the solvent.
2. Use autoclaved deionized / Milli-Q water.
Step 2: Adjust the volume to 10 ml with deionized / Milli-Q water. Mix it again.
Storage
Store the solution in small aliquots (1 ml) at -20°C for a long time.
Applications
A selection marker for bacterial culture in gene cloning experiments.
| Follow the table to prepare a kanamycin solution of different concentrations and volumes. | ||||
| Conc. / Volume | 5 ml | 10 ml | 25 ml | 100 ml |
| 50 mg / ml | 0.25 g | 0.5 g | 1.25 g | 5 g |
| 100 mg / ml | 0.5 g | 1 g | 2.5 g | 10 g |
