T-225 flasks are available from different suppliers in both ventilated and non-ventilated formats. They are useful when you need a large number of cells.
|Available area for cell growth||225 cm2||NA|
|Volume of trypsin (or dissociating agent)||10 ml||7 – 18 ml|
|Volume of culture medium||45 ml||40 – 60 ml|
|Cell number at the time of seeding *||4.5 x 106 cells Ɨ||3.6 – 7.5 x 106 cells|
|Cell number at 100% confluency *||26 x 106 cells||26 – 29 x 106 cells #|
|* for the HeLa cell line|
Ɨ Correspond to seeding density 2 x 104 cells/cm2 (Ref)
# Overconfluent culture after this stage
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