T-175 flasks for cell culture

T-175 flasks are available from different suppliers in both ventilated and non-ventilated formats. They are useful when you need a large number of cells.

Available area for cell growth175 cm2NA
Volume of trypsin (or dissociating agent)8 ml
7 – 14 ml
Volume of culture medium40 ml30 – 50 ml
Cell number at the time of seeding *3.5 x 106 cells Ɨ3.0 – 6.0 x 106 cells
Cell number at 100% confluency *20 x 106 cells 20 – 22 x 106 cells # 
* for the HeLa cell line
Ɨ Correspond to seeding density 2 x 104 cells/cm2 (Ref)
# Overconfluent culture after this stage

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