T-175 flasks are available from different suppliers in both ventilated and non-ventilated formats. They are useful when you need a large number of cells.
| Specifications | Optimum | Range |
| Available area for cell growth | 175 cm2 | NA |
| Volume of trypsin (or dissociating agent) | 8 ml | 7 – 14 ml |
| Volume of culture medium | 40 ml | 30 – 50 ml |
| Cell number at the time of seeding * | 3.5 x 106 cells Ɨ | 3.0 – 6.0 x 106 cells |
| Cell number at 100% confluency * | 20 x 106 cells | 20 – 22 x 106 cells # |
| * for the HeLa cell line Ɨ Correspond to seeding density 2 x 104 cells/cm2 (Ref) # Overconfluent culture after this stage | ||
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