T-25 flasks are available from different suppliers. They are suitable for routing maintenance of a cell line. They are available in both ventilated and non-ventilated formats.
|Available area for cell growth||25 cm2||NA|
|Volume of trypsin (or dissociating agent)||1 ml||1 – 2 ml|
|Volume of culture medium||5 ml||3 – 6 ml|
|Cell number at the time of seeding *||0.5 x 106 cells Ɨ||0.4 – 0.8 x 106 cells|
|Cell number at 100% confluency *||2.8 x 106 cells||2.8 – 3.0 x 106 cells #|
|* for the HeLa cell line|
Ɨ Correspond to seeding density 2 x 104 cells/cm2 (Ref)
# Overconfluent culture after this stage
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