T-25 Flasks for Cell Culture

T-25 flasks are available from different suppliers. They are suitable for routing maintenance of a cell line. They are available in both ventilated and non-ventilated formats. 

Available area for cell growth25 cm2NA
Volume of trypsin (or dissociating agent)1 ml1 – 2 ml
Volume of culture medium5 ml3 – 6 ml
Cell number at the time of seeding *0.5 x 106 cells Ɨ0.4 – 0.8 x 106 cells
Cell number at 100% confluency *2.8 x 106 cells 2.8 – 3.0 x 106 cells # 
* for the HeLa cell line
Ɨ Correspond to seeding density 2 x 104 cells/cm2 (Ref)
# Overconfluent culture after this stage

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