T-75 flasks are available from different suppliers in both ventilated and non-ventilated formats. They are useful when you need a constant supply of a moderate number of cells.
| Specifications | Optimum | Range |
| Available area for cell growth | 75 cm2 | NA |
| Volume of trypsin (or dissociating agent) | 3 ml (Ref) | 2 – 5 ml |
| Volume of culture medium | 15 ml | 10 – 20 ml |
| Cell number at the time of seeding * | 1.5 x 106 cells Ɨ | 1.2 – 2.5 x 106 cells |
| Cell number at 100% confluency * | 8.5 x 106 cells | 8.5 – 9.5 x 106 cells # |
| * for the HeLa cell line Ɨ Corresponds to seeding density 2 x 104 cells/cm2 (Ref) # Overconfluent culture after this stage | ||
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