T-75 flasks are available from different suppliers in both ventilated and non-ventilated formats. They are useful when you need a constant supply of a moderate number of cells.
|Available area for cell growth||75 cm2||NA|
|Volume of trypsin (or dissociating agent)||3 ml (Ref)||2 – 5 ml|
|Volume of culture medium||15 ml||10 – 20 ml|
|Cell number at the time of seeding *||1.5 x 106 cells Ɨ||1.2 – 2.5 x 106 cells|
|Cell number at 100% confluency *||8.5 x 106 cells||8.5 – 9.5 x 106 cells #|
|* for the HeLa cell line|
Ɨ Corresponds to seeding density 2 x 104 cells/cm2 (Ref)
# Overconfluent culture after this stage
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