T-75 Flasks for Cell Culture

T-75 flasks are available from different suppliers in both ventilated and non-ventilated formats. They are useful when you need a constant supply of a moderate number of cells.

Available area for cell growth75 cm2NA
Volume of trypsin (or dissociating agent)3 ml (Ref)2 – 5 ml
Volume of culture medium15 ml10 – 20 ml
Cell number at the time of seeding *1.5 x 106 cells Ɨ1.2 – 2.5 x 106 cells
Cell number at 100% confluency *8.5 x 106 cells 8.5 – 9.5 x 106 cells # 
* for the HeLa cell line
Ɨ Corresponds to seeding density 2 x 104 cells/cm2 (Ref)
# Overconfluent culture after this stage

Was this post helpful?

Author: admin

Leave a Reply

Your email address will not be published. Required fields are marked *