T4 DNA Polymerase

  • Template-dependent DNA polymerase
  • Catalyzes 5′-3′ synthesis from primed single-stranded DNA
  • Possesses 5’→3′ polymerase activity
  • Possesses 3′-5′ exonuclease activity
  • Lacks 5′-3′ exonuclease activity


  • Blunting of DNA ends (fill-in of 5′-overhangs or/and removal of 3′-overhangs)
  • DNA labeling (Probe labeling)

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