- Template-dependent DNA polymerase
- Catalyzes 5′-3′ synthesis from primed single-stranded DNA
- Possesses 5’→3′ polymerase activity
- Possesses 3′-5′ exonuclease activity
- Lacks 5′-3′ exonuclease activity
Applications
- Blunting of DNA ends (fill-in of 5′-overhangs or/and removal of 3′-overhangs)
- DNA labeling (Probe labeling)
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