Tfl DNA Polymerase

  • Isolated from the thermophilic eubacterium Thermus flavus
  • Thermostable DNA polymerase
  • Exhibits a 5′→3′ exonuclease activity
  • Lacks a 3′→5′ exonuclease activity (No proofreading activity)
  • can use RNA as a template in the presence of Mn2+
  • Broader Mg2+ ions concentration tolerance as compared to Taq DNA polymerase


  • Polymerase chain reaction (PCR)

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