- Isolated from the thermophilic eubacterium Thermus flavus
- Thermostable DNA polymerase
- Exhibits a 5′→3′ exonuclease activity
- Lacks a 3′→5′ exonuclease activity (No proofreading activity)
- can use RNA as a template in the presence of Mn2+
- Broader Mg2+ ions concentration tolerance as compared to Taq DNA polymerase
Application
- Polymerase chain reaction (PCR)
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