Tag: Plasmid
Protocol: Double Digestion of Plasmid DNA with FastDigest EcoRV and HindIII Restriction Enzymes
A 100 – 200 ng plasmid DNA is sufficient to analyze by restriction digestion. Here we describe a procedure to double digest plasmid DNA with FastDigest EcoRV and HindIII restriction enzymes (from ThermoFisher Scientific). This procedure can be used to check the presence of a DNA fragment cloned in a vector. Double digestion will release the fragment of the appropriate size.
Protocol: Double Digestion of Plasmid DNA with FastDigest EcoRV and HindIII Restriction Enzymes
A 100 – 200 ng plasmid DNA is sufficient to analyze by restriction digestion. Here we describe a procedure to double digest plasmid DNA with FastDigest EcoRV and HindIII restriction enzymes (from ThermoFisher Scientific). This procedure can be used to check the presence of a DNA fragment cloned in a vector. Double digestion will release the fragment of the appropriate size.