U2AF is an important RNA-splicing factor that helps recognize the 3′ end of many introns. Learn how U2AF65 and U2AF35 interact with the polypyrimidine tract and 3′ splice site and help coordinate spliceosome assembly during pre-mRNA processing.
The polypyrimidine tract is a U- and C-rich sequence near the 3′ end of many introns that helps the spliceosome recognize the 3′ splice site. Learn how it works with the branch point, splicing factors, and other RNA signals to regulate RNA splicing and gene expression.
The branch point is a key sequence region within an intron that contains an important adenosine used during the first reaction of RNA splicing. It helps the spliceosome form the intron lariat and accurately remove introns from pre-mRNA.
Splice-site mutations are genetic variants that disrupt the signals required for accurate RNA splicing. They can cause exon skipping, intron retention, cryptic splice-site activation, abnormal mRNA production, and changes in protein expression.
Cryptic splice sites are normally unused or rarely used splice-like sequences that can become activated when normal splice sites are weakened or when regulatory conditions change. Their activation can alter RNA splicing, mRNA structure, gene expression, and protein production.
Alternative 5′ and 3′ splice sites allow cells to select different boundaries during RNA splicing, producing mature RNA transcripts with different structures. Learn how spliceosomes, splicing factors, and regulatory sequences control alternative splice-site selection and influence gene expression.
Exon skipping is a form of alternative RNA splicing in which one or more exons are excluded from mature mRNA. Learn how splice sites, splicing factors, and regulatory elements control exon inclusion and skipping and how this process affects gene expression and protein diversity.
Splicing enhancers and silencers are regulatory RNA elements that help control which splice sites are used during RNA splicing. By interacting with splicing factors and the spliceosome, they influence exon inclusion, exon skipping, alternative splicing, and gene expression.
Splice sites are RNA sequence signals that guide the spliceosome during intron removal and exon joining. Learn about 5′ and 3′ splice sites, branch points, splicing factors, mutations, and alternative splicing.
Splicing factors are proteins that regulate RNA splicing by influencing splice-site selection and spliceosome activity. Learn how they control alternative splicing, gene expression, development, and disease.
U2AF is an important RNA-splicing factor that helps recognize the 3′ end of many introns. Learn how U2AF65 and U2AF35 interact with the polypyrimidine tract and 3′ splice site and help coordinate spliceosome assembly during pre-mRNA processing.
The polypyrimidine tract is a U- and C-rich sequence near the 3′ end of many introns that helps the spliceosome recognize the 3′ splice site. Learn how it works with the branch point, splicing factors, and other RNA signals to regulate RNA splicing and gene expression.
The branch point is a key sequence region within an intron that contains an important adenosine used during the first reaction of RNA splicing. It helps the spliceosome form the intron lariat and accurately remove introns from pre-mRNA.
Splice-site mutations are genetic variants that disrupt the signals required for accurate RNA splicing. They can cause exon skipping, intron retention, cryptic splice-site activation, abnormal mRNA production, and changes in protein expression.
Cryptic splice sites are normally unused or rarely used splice-like sequences that can become activated when normal splice sites are weakened or when regulatory conditions change. Their activation can alter RNA splicing, mRNA structure, gene expression, and protein production.
Alternative 5′ and 3′ splice sites allow cells to select different boundaries during RNA splicing, producing mature RNA transcripts with different structures. Learn how spliceosomes, splicing factors, and regulatory sequences control alternative splice-site selection and influence gene expression.
Exon skipping is a form of alternative RNA splicing in which one or more exons are excluded from mature mRNA. Learn how splice sites, splicing factors, and regulatory elements control exon inclusion and skipping and how this process affects gene expression and protein diversity.
Splicing enhancers and silencers are regulatory RNA elements that help control which splice sites are used during RNA splicing. By interacting with splicing factors and the spliceosome, they influence exon inclusion, exon skipping, alternative splicing, and gene expression.
Splice sites are RNA sequence signals that guide the spliceosome during intron removal and exon joining. Learn about 5′ and 3′ splice sites, branch points, splicing factors, mutations, and alternative splicing.
Splicing factors are proteins that regulate RNA splicing by influencing splice-site selection and spliceosome activity. Learn how they control alternative splicing, gene expression, development, and disease.
