Terminal Deoxynucleotidyl Transferase (TdT)

  • Template independent DNA polymerase
  • Also known as terminal transferase
  • A member of the X family of DNA polymerase enzymes
  • Add deoxynucleotides to the 3′ hydroxyl terminus of DNA molecules without any template requirement

Applications in molecular biology

  • End labelling and probe preparation
  • Creating T-vector for cloning

REFERENCES

Motea & Berdis, 2010. Terminal deoxynucleotidyl transferase: the story of a misguided DNA polymerase. Biochim Biophys Acta. 1804(5), 1151-1166. PMID-19596089; Full-Text Links: sciencedirect, PMC2846215 (download PDF)

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