Tth DNA polymerase

  • Isolated from the thermophilic eubacterium Thermus thermophilus HB8
  • Optimum reaction temperature: 75 °C
  • Optimum pH: ~9.0 (25 °C)
  • Intrinsic reverse transcriptase (RT) activity
  • 5′-3′ DNA polymerase
  • lacks 3′-5′ exonuclease activity (proofreading)
  • Perform both reverse transcription and DNA amplification at high temperature


  • Polymerase chain reaction (PCR)
  • Labelling oligos and probe preparation

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