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Recommended Agarose Gel Percentage for Efficient Separation of DNA Fragments

Agarose Gel is a porous matrix that acts as a sieve through which negatively charged linear DNA fragments migrate under an…

Molecular Weight and Equivalent Weight of Common Acids and Bases

Chemical name Formula Number of dissociable H+/OH— (valence) in solution Molar mass (= weight weight) g/mol Equivalent weight (g/eq) Hydrochloric…

Cellular Functions of Integrins

Integrins are reported to regulate the following cellular functions. Cell survival and death Cell cycle progression and proliferation Cell shape…

List of Integrin Subunits

α1 integrin subunit  α2 integrin subunit  α3 integrin subunit  α4 integrin subunit  α5 integrin subunit  α6 integrin subunit  α7 integrin…

List of Integrin Family Members

α1β1 integrin  α2β1 integrin  α3β1 integrin  α4β1 integrin  α5β1 integrin  α6β1 integrin  α7β1 integrin  α8β1 integrin  α9β1 integrin  α10β1 integrin …

Integrins in Diseases

Aberrant integrin signaling is reported in the following diseases Thrombosis  Autoimmune diseases  Cancer

Physiological Functions of Integrins

Integrins play pivotal roles in the following physiological processes Wound repair Immunity Hemostasis

Preparation of 6X DNA Loading Dye (Xylene Cyanol FF and Sucrose)

Overview DNA sample is mixed with DNA loading dye prior to loading into the wells of agarose gel.  DNA loading…

Preparation of 6X DNA Loading Buffer (Orange G, Xylene Cyanol FF and Ficoll 400)

A 10 ml of 6X DNA loading dye can be prepared by dissolving and mixing 40 mg orange G, 25 mg xylene cyanol FF, 1.5 g Ficoll 400 and 0.1 ml of 1M Tris.Cl, pH 7.6 in sterile deionized or distilled water to a final volume of 10 ml.

Mobility of Xylene Cyanol in Agarose Gel

% agarose gel Mobility of xylene cyanol corresponding to DNA fragment (bp) in 1X TBE buffer Mobility of xylene cyanol…

Suppliers: Power Supply for Electrophoresis

Note: Power Supply for Electrophoresis can be purchased from any of the following suppliers. You must visit the supplier’s product…

Suppliers: Horizontal Electrophoresis System for Agarose Gel Electrophoresis

Note: Horizontal Electrophoresis System can be purchased from any of the following suppliers. You must visit the supplier’s product homepage…

Electrophoresis

Electrophoresis (Electro: electric, Phoresis (greek word): to carry) is the migration of charged molecules or particles under the influence of an electric field. Electrophoresis-based methods are extensively employed for the analysis, separation and purification of biomolecules such as nucleic acid and proteins in laboratories.

Bromophenol Blue

IUPAC name 4,4′-(1,1-dioxido-3H-2,1-benzoxathiole-3,3-diyl)bis(2,6-dibromophenol) Synonyms 3′,3”,5′,5”-tetrabromophenolsulfonephthalein, BPB, Albutest, Tetrabromphenol Blue Chemical formula C19H10Br4O5S Molar mass 669.96 g/mol Molecular weight 669.96 Odor…

Integrin Ligands

List of integrin ligands Bone sialoprotein (BSP) Collagen (extracellular matrix proteins) Cysteine rich protein 61 (CCN1) Developmental locus-1 (Del-1) Elastin…