Author:

Agarose gel can be used for the separation of nucleic acids (DNA and RNA) and high molecular weight proteins or protein complexes.

Agarose is a useful matrix for a number of analytical and preparative techniques including gel electrophoresis, chromatography, and support matrix to immobilize enzymes and cells. Agarose is available in a variety of forms, which differ in physical properties. The two most common agarose are standard agarose and low melting agarose. Standard agarose is most commonly used for analysis of DNA and RNA. Low melting agarose is often used for preparative purposes such as elution of DNA fragments and complexes.

Reagents> 1M Tris.Cl (pH 8.0) solution, autoclaved> 0.5 EDTA (pH 8.0) solution, autoclaved> Deionized / Milli-Q water Equipment and disposables>…

Molarity of Glacial Acetic Acid

Concentration of Ammonium Hydroxide in % (wt/wt) : % (wt/wt) (Change the % (wt/wt) concentration) Density of Ammonium Hydroxide: g/ml…

Step 1: Calculate the volume of 100 grams of Nitric acid.Formula:Density =  weight / volume orVolume =  weight / density…

Concentration of Formic acid in % (wt/wt) : % (wt/wt) (Change the % (wt/wt) concentration) Density of glacial Formic acid:…