Category: Lab Notes

The molarity and normality of the Sodium hydroxide (NaOH) solution are the same which means 1M NaOH solution is also…

NOTES Sodium Hydroxide (NaOH)  Molarity vs Normality of Sodium Hydroxide (NaOH) Solution SOLUTION PREPARATION Preparation of 10 M Sodium Hydroxide…

As the name suggests, co-immunoprecipitation employs specific antibodies to sequester a protein of interest together with its interacting partners from…

OVERVIEW The 10x Tris-Glycine Transfer Buffer can be used to transfer proteins from polyacrylamide gel to nitrocellulose or PVDF membranes…

Tris base (C4H11NO3) is an organic compound of four elements: Carbon, Hydrogen, Nitrogen, and Oxygen. The molecular weight of Tris base is 121.13574 which can be calculated by adding up the total weight (atomic weight multiplied by their number) of all its elements.

SDS-PAGE running buffer contains Tris Base, Glycine, and sodium dodecyl sulfate (SDS). It is used to run protein in SDS-PAGE…

The normality of Hydrochloric acid is equal to the molarity of Hydrochloric acid which means that a solution of 1M is also a 1N solution of hydrochloric acid.

Amino acid name  One letter symbol Three letters symbol Chemical formula Molecular weight Alanine  A ala C3H7NO2 89.09 Arginine  R…

Bicine (C6H13NO4) is an organic compound of four elements: Carbon, Hydrogen, Nitrogen, and Oxygen. The molecular weight of Bicine is 163.17254 which can be calculated by adding up the total weight (atomic weight multiplied by their number) of all its elements.

A 100 – 200 ng plasmid DNA is sufficient to analyze by restriction digestion. Here we describe a procedure to double digest plasmid DNA with FastDigest EcoRV and HindIII restriction enzymes (from ThermoFisher Scientific). This procedure can be used to check the presence of a DNA fragment cloned in a vector. Double digestion will release the fragment of the appropriate size. 

CAS Number 7681-49-4 Molecular Weight 41.99 InChI 1S/FH.Na/h1H;/q;+1/p-1 InChI key PUZPDOWCWNUUKD-UHFFFAOYSA-M Physical state (Room temperature Solid, powder Colour White

Pyridine (C5H5N) is an organic compound of three elements: Carbon, Hydrogen, and Nitrogen. The molecular weight of Pyridine is 79.10024 which can be calculated by adding up the total weight (atomic weight multiplied by their number) of all its elements.

Sodium molybdate (Na2MoO4) is an inorganic compound of three elements: Sodium, Molybdenum, and Oxygen. The molecular weight of Sodium molybdate is 205.92713856 which can be calculated by adding up the total weight (atomic weight multiplied by their number) of all its elements.

Sodium sulfate (Na2SO4) is an inorganic compound of three elements: Sodium, Sulfur, and Oxygen. The molecular weight of Sodium sulfate is 142.04313856 which can be calculated by adding up the total weight (atomic weight multiplied by their number) of all its elements.

Gastrin-17 Pepsinogens