Category: Lab Notes

Staining PVDF Membrane with Ponceau S after Protein Transfer

Overview: Like nitrocellulose membrane, PVDF membrane can also be stained with Ponceau S stain to reveal protein bands after western…

Ponceau S

Ponceau S belongs to a group of azo dyes that can stain proteins in red/pink color. Chemically it is a…

Ponceau S Staining of Nitrocellulose Membrane after Western Blotting

Overview: Staining of nitrocellulose membrane using Ponceau S is often used to evaluate the quality and transfer efficiency of western…

Preparation of Ponceau S Staining Solution

Overview: Ponceau S stain is often used to evaluate the transfer efficiency of western blotting. It reversibly stains the protein…

Cell/Tissue Culture Vessels: Flasks, Plates and Dishes

A variety of cell/tissue culture vessels are available commercially and can be purchased from various suppliers. These vessels are specifically…

Transfection

Transfection is a very common and powerful method to deliver nucleic acid (DNA/RNA) into tissue culture cells. Usually, the purpose…

Molarity of 32% (w/w) Hydrochloric Acid (HCl)

The molarity of 32% (w/w) Hydrochloric Acid is 10.181 M. OVERVIEW A 32% (w/w) Hydrochloric Acid is a clear colorless…

Topic: Hydrochloric acid (HCl)

NOTES Hydrogen Chloride (HCl) Hydrochloric Acid  Normality vs Molarity of Hydrochloric Acid CALCULATIONS Hydrogen Chloride (HCl) Molecular Weight Calculation     Molarity…

Preparation of 1M Acetic Acid Solution from Glacial Acetic Acid

Overview: A 1M Acetic acid solution can be prepared by diluting the concentrated acetic acid stock solution. Glacial acetic acid,…

Topic: Acetic Acid

ChemDB: Acetic Acid Acetic Acid (CH3COOH) Molecular Weight Calculation Suppliers: Glacial Acetic Acid How to Calculate Molarity of Glacial Acetic…

H-1152

ROCK inhibitor

Y-27632

Cell-permeable Highly selective and potent inhibitor of Rho-associated protein kinases ROCK1 (Ki = 220 nM) and ROCK2 (Ki = 300…

List of Epithelial-specific Integrins

The following integrins are considered specific to epithelial cells. α1β1 α2β1  α3β1 α5β1 α6β1  α9β1  αvβ5  αvβ6  α6β4

List of Type II Classical Cadherins

VE-cadherin (Cdh5) K-cadherin (Cdh6/6B) Cdh7 Cdh8 T1-cadherin (Cdh9) T2-cadherin (Cdh10) OB-cadherin (Cdh11) N-cadherin 2 (Cdh12) Cdh18 Cdh19 MN-cadherin (Cdh20) PB-cadherin…