Category: Lab Notes

Endonuclease Type IIS Restriction enzyme Cleaves double-stranded DNA outside of specific recognition site Recognizes asymmetric DNA sequences (see fig) DNA…

Endonuclease Type IIS Restriction enzyme Cleaves double-stranded DNA outside of specific recognition site Recognizes asymmetric DNA sequences (see fig) DNA…

Endonuclease Type IIS Restriction enzyme Cleaves double-stranded DNA outside of specific recognition site Recognizes asymmetric DNA sequences (see fig) DNA…

A 68kDa C-terminal fragment of E. coli DNA Polymerase I Produced by proteolytic cleavage of E. coli DNA polymerase I…

Isolated from the thermophilic eubacterium Thermus thermophilus HB8 Optimum reaction temperature: 75 °C Optimum pH: ~9.0 (25 °C) Intrinsic reverse…

Template-dependent DNA polymerase Catalyzes 5′-3′ synthesis from primed single-stranded DNA Possesses 5’→3′ polymerase activity Possesses 3′-5′ exonuclease activity Lacks 5′-3′…

Isolated from the thermophilic eubacterium Thermus flavus Thermostable DNA polymerase Exhibits a 5′→3′ exonuclease activity Lacks a 3′→5′ exonuclease activity…

Isolated from the thermophilic bacterium Thermus aquaticus Thermostable DNA polymerase Catalyzes 5’→3′ DNA synthesis (5’→3′ polymerase activity) from primed single-stranded…

OVERVIEW Can withstand high temperature Active at high temperature Important tools for polymerase chain reaction Examples of Thermostable DNA Polymerases…

OVERVIEW A 48% (w/w) Hydrofluoric acid can be purchased from several commercial suppliers. It is a clear colorless liquid and…

OVERVIEW A 48% (w/w) Hydrofluoric acid (HF) is a clear colorless aqueous solution of Hydrogen fluoride (HF) gas. It can…

Note: 48% (w/w) Hydrofluoric acid (HF) can be purchased from the following suppliers. You must visit the supplier’s product page…

Endonuclease Type IIS Restriction enzyme Cleaves double-stranded DNA outside of specific recognition site Recognizes asymmetric DNA sequences (see fig) DNA…

OVERVIEW A 70% (w/w) perchloric acid can be purchased from several commercial suppliers. It is a clear colorless liquid and…

OVERVIEW A 70% (w/w) Perchloric acid is a clear colorless aqueous solution of HClO4. It can be purchased from many…