- Synonyms: HEK293A GFP-LC3
- Keywords: HEK293-A cells (subclone of HEK-293) stably expressing GFP-tagged rat LC3
- Description:
- Parent cell line: 293 (also known as HEK-293)
- Immediate parent: HEK-293A
- HEK293-A cells were transfected with GFP-tagged rat LC3 and selected in G418. This is a clonal cell line stably express GFP-tagged rat LC3
- Culture Type: Cell line
- Origin/Source:
- Organism:
- Species: Human
- Age: Fetus
- Tissue of Origin: Embryonic Kidney
- Organism:
- Cell line features:
- Morphology:
- Cell Type: Neuronal (culturecollections)
- Growth Mode: Adherent
- Morphology:
- Applications:
- Reporter cell line, It can be used to monitor induction of autophagy after amino acid starvation or rapamycin treatment.
- Handling and maintenance:
- Biosafety level: BSL 2 (It may differ in your country, follow the guidelines of your institute/country)
- GMO Status: GMO1
- Complete culture medium:
- DMEM + 2mM Glutamine + 10% FBS + G418 (400 µg/ml)
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 10% CO₂, 90% Air (culturecollections)
- Subculturing:
- Cell dissociation procedure: 0.05% Trypsin/EDTA
- Harvesting time: 80% confluence culture
- Subcultivation Ratio:
- Medium Renewal:
- Seeding densities: 2×10⁴ cells/cm²
- Cell dissociation procedure: 0.05% Trypsin/EDTA
- Cryopreservation
- Growth medium + 20% FBS + 10% DMSO
- Cryopreservation temperature: Liquid nitrogen gas phase or -150°C freezer
- Availability:
- Culture Collections (UK Health Security Agency): Product link
- CellBank Australia: Product Link
- References:
- https://www.culturecollections.org.uk/nop/product/hek293a-gfp-lc3
- Kochl et al., 2006. Microtubules facilitate autophagosome formation and fusion of autophagosomes with endosomes. Traffic. 7(2), 129-45. PMID-16420522; Full-Text Links: Wiley (Download PDF)
- Chan et al., 2009. Kinase-inactivated ULK proteins inhibit autophagy via their conserved C-terminal domains using an Atg13-independent mechanism. Mol Cell Biol. 29(1), 157-171. PMID-18936157; Full-Text Links: Tandfonline, PMC (Download PDF)
- Abbreviations
- DMEM (Dulbecco’s Modified Eagle’s Medium)
- FBS (Foetal Bovine Serum)
- GMO1 (Genetically Modified Organism Class 1)
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