- Synonyms: 293TT
- Description:
- Parent cell line: 293 (also known as HEK-293)
- Immediate parent: 293T
- cDNA encoding for SV40 spliced T antigen was incorporated in 293T cell line in the presence of 250 µg/mL of hygromycin and clones were isolated and later tested for their ability to produce papillomavirus pseudovirion.
- Keywords:
- Culture Type: Cell line
- Origin/Source:
- Organism:
- Species: Human (Homo sapiens)
- Age: Fetus
- Tissue of Origin: Embryonic Kidney
- Organism:
- Cell line features:
- Morphology: Epithelial-like
- Cell Type: Epithelial
- Growth Mode: Adherent
- Morphology: Epithelial-like
- Applications:
- Bioproduction
- Production of papillomavirus and polyomavirus-based reporter vectors, as well as propagation of torque teno viruses, BK and JC polyomavirus primary isolates.
- Handling and maintenance
- Biosafety level: BSL 2 (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- DMEM + 1% L-glutamine + 1% Non-Essential Amino Acids (ThermoFisher Scientific # 11140-050) + 250 µg/mL Hygromycin B + 10% heat-inactivated FBS
- DMEM + 1% L-glutamine + 1% Non-Essential Amino Acids (ThermoFisher Scientific # 11140-050) + 250 µg/mL Hygromycin B + 10% heat-inactivated FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO₂, 95% Air
- Subculturing:
- Cell dissociation procedure: 0.05% (w/v) Trypsin – 0.53 mM EDTA solution
- Harvesting time: 80% confluence culture
- Subcultivation Ratio: 1:3 to 1:8
- Medium Renewal: Every 2 to 3 days
- Seeding densities: 5.0 X 104 cell/cm2
- Cell dissociation procedure: 0.05% (w/v) Trypsin – 0.53 mM EDTA solution
- Cryopreservation
- Complete culture medium + 5% DMSO
- Cryopreservation temperature: Liquid nitrogen gas phase or -150°C freezer
- Availability:
- American Type Culture Collection (ATCC): Product link
- References:
- https://www.atcc.org/products/crl-3467
- Buck et al., 2004. Efficient intracellular assembly of papillomaviral vectors. J Virol 78(2): 751-7, 2004. PMID-14694107; Full-Text Links: ASM, PMC
- Abbreviations
- DMEM (Dulbecco’s Modified Eagle’s Medium)
- FBS (Fetal Bovine Serum)
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