Cell Line: K562 AZQR

  • Synonyms
    • K-562 AZQR
    • K562 AZQR
  • Culture Type: Cell line 
  • Keywords: Human chronic myeloid leukemia in blast crisis 
  • Source: K562 AZQR is a subclone of K562 cell line, developed by treatment with AZQ. K562 AZQR cells are resistant to AZQ (Ward et al., 1995).
    • Organism: Human (Homo sapiens), 53-year-old caucasian female
    • Tissue of Origin: blood
    • Tissue: Bone; Marrow, pleural effusion
    • Disease: Chronic myeloid leukemia in blast crisis
  • Cell line features:
    • Morphology: round and large, single cells
    • Cell Type: Lymphoblast
    • Growth Mode: Suspension
    • Life span: Infinite, continuous culture
    • Tumorigenicity: tumorigenic in nude mice
  • Gene expression
  • These cells express decreased levels of cytochrome P-450 reductase and DT-diaphorase and increased levels of glutathione and superoxide dismutase, compared to the parent cell line (K562) (Ward et al., 1995).
  • Carry Philadelphia chromosome with a BCR-ABL fusion gene
  • Drug resistance
  • Handling
    • Biosafety Information: BSL 2 (Culturecollections) (It may differ in your country, follow the guidelines of your institute/country)
    • Complete culture medium: 
      • RPMI 1640 (2mM Glutamine) + 10% FBS. Challenge with 1 mM AZQ every 4th passage. 
    • Culture conditions
      • Temperature: 37°C 
      • Atmosphere: 95% Air, 5% CO2 
    • Subculturing procedure: Dilution (addition or replacement of fresh culture medium)
      • Maintain cultures between 2 x 105 viable cells/mL to 1 x 106 cells/mL
      • Medium Renewal: Every 2 to 3 days
      • Split ratio: 1:3 to 1:5 every 3 days
    • Cryopreservation
      • Storage conditions: Vapor phase of liquid nitrogen
      • Freezing medium: Complete growth medium + 5% (v/v) DMSO (without AZQ)
  • References: 
  • Abbreviations:
    • AZQ: 2,5-bis(carboethoxyamino)-3,6-diaziridinyl-1,4-benzoquinone
    • MNNG: N-methyl-N’-nitro-N-nitrosoguanidine

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