- Synonyms
- K-562 AZQR
- K562 AZQR
- Culture Type: Cell line
- Keywords: Human chronic myeloid leukemia in blast crisis
- Source: K562 AZQR is a subclone of K562 cell line, developed by treatment with AZQ. K562 AZQR cells are resistant to AZQ (Ward et al., 1995).
- Organism: Human (Homo sapiens), 53-year-old caucasian female
- Tissue of Origin: blood
- Tissue: Bone; Marrow, pleural effusion
- Disease: Chronic myeloid leukemia in blast crisis
- Cell line features:
- Morphology: round and large, single cells
- Cell Type: Lymphoblast
- Growth Mode: Suspension
- Life span: Infinite, continuous culture
- Tumorigenicity: tumorigenic in nude mice
- Gene expression
- These cells express decreased levels of cytochrome P-450 reductase and DT-diaphorase and increased levels of glutathione and superoxide dismutase, compared to the parent cell line (K562) (Ward et al., 1995).
- Carry Philadelphia chromosome with a BCR-ABL fusion gene
- Drug resistance
- AZQ (Ward et al., 1995)
- Adriamycin (Ward et al., 1995)
- Mitozolamide (Ward et al., 1995)
- MNNG (Ward et al., 1995)
- Mitomycin C (Ward et al., 1995)
- Handling
- Biosafety Information: BSL 2 (Culturecollections) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- RPMI 1640 (2mM Glutamine) + 10% FBS. Challenge with 1 mM AZQ every 4th passage.
- Culture conditions
- Temperature: 37°C
- Atmosphere: 95% Air, 5% CO2
- Subculturing procedure: Dilution (addition or replacement of fresh culture medium)
- Maintain cultures between 2 x 105 viable cells/mL to 1 x 106 cells/mL
- Medium Renewal: Every 2 to 3 days
- Split ratio: 1:3 to 1:5 every 3 days
- Cryopreservation
- Storage conditions: Vapor phase of liquid nitrogen
- Freezing medium: Complete growth medium + 5% (v/v) DMSO (without AZQ)
- References:
- Ward et al., 1995. Cross-resistance studies on two K562 sublines resistant to diaziridinylbenzoquinones. Biochem Pharmacol. 50(4), 459-464. PMID-7646550; Full-Text Links: Sciencedirect
- Culturecollections
- Abbreviations:
- AZQ: 2,5-bis(carboethoxyamino)-3,6-diaziridinyl-1,4-benzoquinone
- MNNG: N-methyl-N’-nitro-N-nitrosoguanidine
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